THE REB1 SITE IS AN ESSENTIAL COMPONENT OF A TERMINATOR FOR RNA POLYMERASE-I IN SACCHAROMYCES-CEREVISIAE
THE REB1 SITE IS AN ESSENTIAL COMPONENT OF A TERMINATOR FOR RNA POLYMERASE-I IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/mcb.13.1.649
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发表时间：
1993-01-01
影响因子：
5.3
通讯作者：
REEDER, RH
中科院分区：
文献类型：
--
作者：
LANG, WH;REEDER, RH
我们在酿酒酵母 rRNA 编码基因中鉴定出了 RNA 聚合酶 I 转录的终止子。该终止子位于成熟 25S rRNA 3' 端下游 108 bp 处，与之前研究的脊椎动物聚合酶 I 终止子具有多个特征。例如，酵母终止子是方向依赖性的，被其自身序列抑制，并在必需蛋白结合位点上游17+/-2bp处形成RNA 3'末端。 用于结合先前克隆的REB1蛋白的识别序列(Q.Ju，B.E.Morrow，和J.R.Warner，Mol.Cell.Biol.10：5226-5234，1990)是终止子的重要组成部分。此外，终止效率取决于在 REB1 位点上游延伸至少 12 bp 的序列上下文。
We have identified a terminator for transcription by RNA polymerase I in the genes coding for rRNA of the Yeast Saccharomyces cerevisiae. The terminator is located 108 bp downstream of the 3' end of the mature 25S rRNA and shares several characteristics with previously studied polymerase I terminators in the vertebrates. For example, the yeast terminator is orientation dependent, is inhibited by its own sequence, and forms RNA 3' ends 17 +/- 2 bp upstream of an essential protein binding site. The recognition sequence for binding of the previously cloned REB1 protein (Q. Ju, B. E. Morrow, and J. R. Warner, Mol. Cell. Biol. 10:5226-5234,1990) is an essential component of the terminator. In addition, the efficiency of termination depends upon sequence context extending at least 12 bp upstream of the REB1 site.