Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives
Microarray-based gene expression analysis of strong seed dormancy in rice cv. N22 and less dormant mutant derivatives
复制标题
基于微阵列的水稻种子强休眠基因表达分析。
DOI：
10.1016/j.plaphy.2015.12.001
复制
发表时间：
2016-02-01
影响因子：
6.5
通讯作者：
Wan, Jianmin
中科院分区：
文献类型：
--
作者：
Wu, Tao;Yang, Chunyan;Wan, Jianmin
水稻种子休眠性是水稻抗穗发芽的重要性状。为了了解种子休眠的分子机制，通过转录组分析研究了强休眠品种N22及其弱休眠突变体Q4359和Q4646在抽穗后24天（DAH）的种子的基因表达。微阵列数据显示，与N22相比，Q4359中的差异表达基因多于Q4646中的差异表达基因。在Q4646和N22之间存在差异的大多数基因在Q4359和N22之间也存在差异。对Q4359和Q4646中差异表达的基因的GO分析显示，相对于N22，Q4359和Q4646种子中的一些基因如淀粉生物合成基因被抑制，而代谢基因如碳水化合物代谢基因被增强。参与细胞氧化还原稳态和染色质重塑的一些基因的表达仅在Q4359和N22之间有显著差异。结果表明，细胞氧化还原稳态、染色质重构与种子休眠密切相关。此外，一些参与阿坝信号转导的基因表达下调，一些参与GA生物合成和信号转导的基因表达上调。这些观察结果表明，减少Q4359种子休眠调节阿坝GA拮抗作用。在qSdn-1和qSdn-5区域发现了一些差异表达基因，推测它们可能是种子休眠的候选基因。本研究为进一步研究N22种子休眠机制和克隆种子休眠基因提供了有用的线索。(C)2015年Elsevier Masson SAS。All rights reserved.
Seed dormancy in rice is an important trait related to the pre-harvest sprouting resistance. In order to understand the molecular mechanisms of seed dormancy, gene expression was investigated by transcriptome analysis using seeds of the strongly dormant cultivar N22 and its less dormant mutants Q4359 and Q4646 at 24 days after heading (DAH). Microarray data revealed more differentially expressed genes in Q4359 than in Q4646 compared to N22. Most genes differing between Q4646 and N22 also differed between Q4359 and N22. GO analysis of genes differentially expressed in both Q4359 and Q4646 revealed that some genes such as those for starch biosynthesis were repressed, whereas metabolic genes such as those for carbohydrate metabolism were enhanced in Q4359 and Q4646 seeds relative to N22. Expression of some genes involved in cell redox homeostasis and chromatin remodeling differed significantly only between Q4359 and N22. The results suggested a close correlation between cell redox homeostasis, chromatin remodeling and seed dormancy. In addition, some genes involved in ABA signaling were down-regulated, and several genes involved in GA biosynthesis and signaling were up regulated. These observations suggest that reduced seed dormancy in Q4359 was regulated by ABA GA antagonism. A few differentially expressed genes were located in the regions containing qSdn-1 and qSdn-5 suggesting that they could be candidate genes underlying seed dormancy. Our work provides useful leads to further determine the underling mechanisms of seed dormancy and for cloning seed dormancy genes from N22. (C) 2015 Elsevier Masson SAS. All rights reserved.