Electron microscopic studies on the localization of sodium ions and sodium-potassium-activated adenosinetriphosphatase in chloride cells of eel gills.
Electron microscopic studies on the localization of sodium ions and sodium-potassium-activated adenosinetriphosphatase in chloride cells of eel gills.
复制标题
电镜研究鳗鱼鳃氯细胞中钠离子和钠钾激活的三磷酸腺苷酶的定位。
DOI：
10.1016/0014-4827(70)90613-0
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发表时间：
1970
影响因子：
3.7
通讯作者：
S. Utida
中科院分区：
文献类型：
--
作者：
V. Mizuhira;T. Amakawa;S. Yamashina;N. Shirai;S. Utida
材料与方法采用日本鳗鲡（Anguillu japonica, 120 ~ 150 g）养殖，适应海水（盐度35 g） 1个月。这些动物被斩首，鳃丝被移除，在去离子水中迅速冲洗，并在滤纸上涂抹。钠离子的检测遵循Amakawa & Mizuhira[4]的程序。用2 vol 2%焦硝膦酸钾和1 vol 4%四氧化锇的混合物在WC下固定灯丝1 h；加入少量2%焦锑酸钾调节pH至7.2。然后用乙醇和em-使组织脱水
MATERIALS AND METHODSJapanese cultured eels, Anguillu japonica (120-150 g) adapted to sea water (salinity, 35 g) for 1 month were used. These animals were sacrified by decapitation and the gill filaments were removed, quickly rinsed in deionized water and blotted on filter naner. Sodium ions were detected following the nrocedure of Amakawa & Mizuhira [4]. The-filaments were fixed for 1 h at WC with the mixture of 2 vol of 2% potassium pyroantirnonate and 1 vol of 4% osmium tetroxide; pH was adjusted to 7.2 by adding a small volume of 2% potassium pyroantimonate. The tissues were then dehydrated with ethanol and em-