Quantification of Myocyte Disarray in Human Cardiac Tissue.
Quantification of Myocyte Disarray in Human Cardiac Tissue.
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DOI：
10.3389/fphys.2021.750364
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发表时间：
2021
影响因子：
4
通讯作者：
Sacconi L
中科院分区：
文献类型：
--
作者：
Giardini F;Lazzeri E;Vitale G;Ferrantini C;Costantini I;Pavone FS;Poggesi C;Bocchi L;Sacconi L
正确的三维（3D）-心肌细胞的方向是重要的，在心肌中的有效的张力产生。心脏疾病可引起心脏中的严重重塑过程，例如细胞失调，其可影响器官的电和机械功能。到目前为止，缺乏一种经过验证的方法来映射和量化大规模样本中的肌细胞紊乱。在这项研究中，我们提出了一个实验管道来重建和分析大量样本中的3D心肌细胞结构。我们采用组织透明化、染色和先进的显微镜技术，以微米级分辨率检测相对较大的人类心肌条中的肌节。利用Z-波段周期性的频率分析方法提取三维肌丝方向，提供一个方向图，用于表征在不同的空间尺度的组织结构。作为原理证明，我们将所提出的方法应用于健康和病理重塑的人类心脏组织条。初步结果表明该方法的可靠性：来自健康供体的条带的特征在于组织良好，其中局部混乱是对数正态分布的，并且略微依赖于分析的空间尺度;相反，病理条带显示出明显的组织混乱，其特征在于局部混乱显著依赖于分析的空间尺度。一个虚拟的样品发生器的开发链接这种多尺度的混乱分析与底层的细胞架构。这种方法使我们能够根据3D肌细胞角分散定量评估组织组织结构，并可能为基于细胞分辨率的结构数据开发新的预测模型铺平道路。
Proper three-dimensional (3D)-cardiomyocyte orientation is important for an effective tension production in cardiac muscle. Cardiac diseases can cause severe remodeling processes in the heart, such as cellular misalignment, that can affect both the electrical and mechanical functions of the organ. To date, a proven methodology to map and quantify myocytes disarray in massive samples is missing. In this study, we present an experimental pipeline to reconstruct and analyze the 3D cardiomyocyte architecture in massive samples. We employed tissue clearing, staining, and advanced microscopy techniques to detect sarcomeres in relatively large human myocardial strips with micrometric resolution. Z-bands periodicity was exploited in a frequency analysis approach to extract the 3D myofilament orientation, providing an orientation map used to characterize the tissue organization at different spatial scales. As a proof-of-principle, we applied the proposed method to healthy and pathologically remodeled human cardiac tissue strips. Preliminary results suggest the reliability of the method: strips from a healthy donor are characterized by a well-organized tissue, where the local disarray is log-normally distributed and slightly depends on the spatial scale of analysis; on the contrary, pathological strips show pronounced tissue disorganization, characterized by local disarray significantly dependent on the spatial scale of analysis. A virtual sample generator is developed to link this multi-scale disarray analysis with the underlying cellular architecture. This approach allowed us to quantitatively assess tissue organization in terms of 3D myocyte angular dispersion and may pave the way for developing novel predictive models based on structural data at cellular resolution.
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心房颤动和心力衰竭病理生理学中的钙。
影响因子：
4
作者：
Denham NC;Pearman CM;Caldwell JL;Madders GWP;Eisner DA;Trafford AW;Dibb KM
通讯作者：
Dibb KM
DOI：
10.1186/s12968-015-0129-x
发表时间：
2015-04-29
期刊：
Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance
影响因子：
--
作者：
Bernus O;Radjenovic A;Trew ML;LeGrice IJ;Sands GB;Magee DR;Smaill BH;Gilbert SH
通讯作者：
Gilbert SH
影响因子：
14
作者：
Feinberg, Adam W.;Alford, Patrick W.;Jin, Hongwei;Ripplinger, Crystal M.;Werdich, Andreas A.;Sheehy, Sean P.;Grosberg, Anna;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
影响因子：
4
作者：
Coppini, Raffaele;Ferrantini, Cecilia;Cerbai, Elisabetta
通讯作者：
Cerbai, Elisabetta
影响因子：
6.4
作者：
Campanale, Cosimo M.;Scherrer, Benoit;Sanders, Stephen P.
通讯作者：
Sanders, Stephen P.