Long-chain acyl-CoA esters as indicators of lipid metabolism and insulin sensitivity in rat and human muscle
Long-chain acyl-CoA esters as indicators of lipid metabolism and insulin sensitivity in rat and human muscle
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DOI：
10.1152/ajpendo.2000.279.3.e554
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发表时间：
2000-09-01
影响因子：
5.1
通讯作者：
Cooney, GJ
中科院分区：
文献类型：
--
作者：
Ellis, BA;Poynten, A;Cooney, GJ
长链酰基辅酶A（LCACoA）是一种活化的脂质物质，是脂质代谢中的关键代谢物;它们也在调节其他细胞过程中发挥作用。然而，很少有研究将大鼠和人类肌肉中的LCACoA含量与营养状况和胰岛素作用的变化联系起来。禁食大鼠18 h显著升高了肌肉中三种主要的LCACoA种类（P< 0.001），而高脂喂养红花油（18：2）饮食的大鼠产生胰岛素抵抗，并通过特异性增加18：2-CoA来增加总LCACoA含量（P< 0.0001）。大鼠红肌LCACoA含量（4-8 nmol/g）比脂肪组织（0.4-0.9 nmol/ g，P< 0.001）高4- 10倍，表明肌肉样品中脂肪细胞的任何污染对肌肉LCACoA含量的贡献很小。在人体中，17名男性受试者的肌肉LCACoA含量与全身胰岛素作用的测量值显著相关（r（2）= 0.34，P = 0.01），支持肌肉脂质代谢与胰岛素作用之间的联系。这些结果表明，LCACoA池反映了肌肉中的脂质代谢和营养状态。我们的结论是，肌肉的LCACoA含量提供了一个直接的细胞内脂质代谢和胰岛素作用，这与甘油三酯含量不同，是不受污染的密切相关的脂肪组织的联系指数。
Long-chain acyl-CoAs (LCACoA) are an activated lipid species that are key metabolites in lipid metabolism; they also have a role in the regulation of other cellular processes. However, few studies have linked LCACoA content in rat and human muscle to changes in nutritional status and insulin action. Fasting rats for 18 h significantly elevated the three major LCACoA species in muscle (P< 0.001), whereas high-fat feeding of rats with a safflower oil (18: 2) diet produced insulin resistance and increased total LCACoA content (P< 0.0001) by specifically increasing 18:2-CoA. The LCACoA content of red muscle from rats (4-8 nmol/g) was 4- to 10-fold higher than adipose tissue (0.4-0.9 nmol/ g, P< 0.001), suggesting that any contamination of muscle samples with adipocytes would contribute little to the LCACoA content of muscle. In humans, the LCACoA content of muscle correlated significantly with a measure of whole body insulin action in 17 male subjects (r(2) = 0.34, P = 0.01), supporting a link between muscle lipid metabolism and insulin action. These results demonstrate that the LCACoA pool reflects lipid metabolism and nutritional state in muscle. We conclude that the LCACoA content of muscle provides a direct index of intracellular lipid metabolism and its links to insulin action, which, unlike triglyceride content, is not subject to contamination by closely associated adipose tissue.