The Anti-Inflammatory Effects of Iberin on TNF-α-Stimulated Human Oral Epithelial Cells: In Vitro Research.
The Anti-Inflammatory Effects of Iberin on TNF-α-Stimulated Human Oral Epithelial Cells: In Vitro Research.
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DOI：
10.3390/biomedicines10123155
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发表时间：
2022-12-06
期刊：
影响因子：
4.7
通讯作者：
Hosaka, Keiichi
中科院分区：
文献类型：
--
作者：
Hosokawa, Yoshitaka;Hosokawa, Ikuko;Shimoyama, Masahiro;Fujii, Ayumi;Sato, Juri;Kadena, Kimitake;Ozaki, Kazumi;Hosaka, Keiichi
伊比利亚是一种在十字花科植物中发现的生物活性化学物质，已被证明具有抗癌特性。然而，目前还没有关于其对牙周驻留细胞的影响的报道，许多问题仍然没有答案。本研究的目的是检查伊贝林是否对人口腔上皮细胞具有抗炎作用，包括对TNF-α-στ μ π ι λατεd χε λσ信号转导通路激活的影响。Iberin抑制IL-6和C-X-C基序趋化因子配体10（CXCL 10）的产生，以及血管细胞粘附分子（VCAM）-1、诱导型一氧化氮合酶（iNOS）和环氧合酶（考克斯）-2在肿瘤坏死因子（TNF）-α刺激的人口腔上皮细胞系TR 146细胞中的表达。此外，伊贝林给药增加了抗氧化信号通路的表达，如血红素加氧酶（HO）-1和NAD（P）H醌脱氢酶1（NQO 1）。此外，我们还发现，在TNF-α刺激的TR 146细胞中，伊贝林可抑制核因子（NF）-κB、信号转导和转录激活因子（STAT）3和p70 S6激酶（p70 S6 K）-S6核糖体蛋白（S6）通路的激活。总之，伊贝林通过阻止特定信号转导通路的激活来减少人类口腔上皮细胞中炎症介质的表达。
Iberin is a bioactive chemical found in cruciferous plants that has been demonstrated to have anticancer properties. However, there have been no reports on its effects on periodontal resident cells, and many questions remain unanswered. The aim of this study was to examine whether iberin had anti-inflammatory effects on human oral epithelial cells, including influences on signal transduction pathway activation in TNF-α-στιμυλατεd χελλσ. Iberin inhibited the production of interleukin (IL)-6 and C-X-C motif chemokine ligand 10 (CXCL10), as well as the expression of vascular cell adhesion molecule (VCAM)-1, inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 in tumor necrosis factor (TNF)-α-stimulated TR146 cells, a human oral epithelial cell line. Moreover, iberin administration increased the expression of antioxidant signaling pathways, such as Heme Oxygenase (HO)-1 and NAD(P)H quinone dehydrogenase 1 (NQO1). Furthermore, we found that iberin could inhibit the activation of the nuclear factor (NF)-κB, signal transducer and activator of transcription (STAT)3, and p70S6 kinase (p70S6K)-S6 ribosomal protein (S6) pathways in TNF-α-stimulated TR146 cells. In conclusion, iberin reduced inflammatory mediator expression in human oral epithelial cells by preventing the activation of particular signal transduction pathways.
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影响因子：
7.3
作者：
通讯作者：
--
影响因子：
3.1
作者：
Shimoyama, Masahiro;Hosokawa, Yoshitaka;Hosokawa, Ikuko;Ozaki, Kazumi;Hosaka, Keiichi
通讯作者：
Hosaka, Keiichi
影响因子：
5.6
作者：
Medapati MR;Bhagirath AY;Singh N;Schroth RJ;Bhullar RP;Duan K;Chelikani P
通讯作者：
Chelikani P
DOI：
10.3390/bioengineering9090470
发表时间：
2022-09-14
期刊：
Bioengineering (Basel, Switzerland)
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.8
作者：
Medapati, Manoj Reddy;Singh, Nisha;Chelikani, Prashen
通讯作者：
Chelikani, Prashen