The Trk family of tyrosine kinases: receptors for NGF-related neurotrophins.
The Trk family of tyrosine kinases: receptors for NGF-related neurotrophins.
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酪氨酸激酶 Trk 家族：NGF 相关神经营养素的受体。
DOI：
10.1101/sqb.1992.057.01.006
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Soppet,D
中科院分区：
文献类型：
--
作者：
Parada,LF;Tsoulfas,P;Tessarollo,L;Blair,J;Reid,SW;Soppet,D
方法材料。WGA-琼脂糖6 B和蛋白A-琼脂糖4 B来自Pharmacia（皮斯卡特维，新泽西）。碱性磷酸酶偶联的抗小鼠和抗兔抗体、BCIP和硝基蓝四唑来自Promega Biotec（麦迪逊，威斯康星州）。在NCI-FCRDC生成肽。在NCI-FCRDC或Cocalico Biologicals（雷姆斯敦，宾夕法尼亚州）制备抗血清。Dulbecco改良Eagle培养基（DMEM），Leibovitz L-15
METHODSMaterials. WGA-Sepharose 6B and Protein A-Sepharose 4B were from Pharmacia (Piscataway, New Jersey). Alkaline phosphatase-conjugated anti-mouse and anti-rabbit antibodies, BCIP, and nitro-blue tetrazolium were from Promega Biotec (Madison, Wisconsin). Peptides were generated at NCI-FCRDC. Antisera were prepared at NCI-FCRDC or by Cocalico Biologicals (Reamstown, Pennsylvania). Dulbecco's modified Eagle's medium (DMEM), Leibovitz L-15