PROSTANOIDS IN CORTICAL SUBARACHNOID CEREBROSPINAL-FLUID AND PIAL ARTERIAL DIAMETER IN NEWBORN PIGS
PROSTANOIDS IN CORTICAL SUBARACHNOID CEREBROSPINAL-FLUID AND PIAL ARTERIAL DIAMETER IN NEWBORN PIGS
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DOI：
10.1161/01.res.57.5.689
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发表时间：
1985-01-01
影响因子：
20.1
通讯作者：
BUSIJA, DW
中科院分区：
文献类型：
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作者：
LEFFLER, CW;BUSIJA, DW
本研究旨在探讨氯醛糖麻醉、血气和pH正常的新生猪以及低氧高二氧化碳复合状态下脑前列腺素合成与软脑膜动脉管径的关系。不到5日龄的仔猪配备了关闭的颅窗，以便直接观察软脑膜血管，应用前列腺素E_2，并采集皮质蛛网膜下腔脑脊液。我们发现前列腺素类物质聚集在皮质表面的脑脊液中。在动脉血中唯一检测到的前列腺素是6-酮-前列腺素F1α。[442.+-.74pg/ml(放射免疫法)]。只有少量的6-酮-前列腺素F1α。(214.+-。53 pg/ml)和血栓素B_2(122±-)。在枕大池脑脊液中检出18 pg/ml)。较高浓度的6-酮-前列腺素F1α。(1056.+-)。159 pg/ml)，血栓素B_2(229±-)。Pg/ml)和前列腺素E_2(4235±-)。269 pg/ml)。与动脉和脑池中6-酮-前列腺素F_1α、血栓素B_2和前列腺素E_2浓度相反，在9%二氧化碳、10%氧气、(6-酮-前列腺素F_1α，5436±-)的情况下，皮质蛛网膜下腔液中6-酮-前列腺素F_1α、血栓素B_2和前列腺素E_2的浓度是可逆的。1576 pg/ml；血栓素B2,694。+-。122pg/ml，前列腺素E_2，12,455.+-。3688pg/ml)。此外，局部应用前列腺素E_2后，软脑膜动脉扩张，其浓度与低氧和高碳酸血症合并时蛛网膜下液中的前列腺素E_2浓度相同。全身注射三水吲哚美辛(5 mg/kg)可显著降低蛛网膜下腔皮质液中前列腺素浓度，减轻缺氧高碳酸血症所致的软脑膜动脉扩张作用。我们的数据与大脑前列腺素是新生猪脑血流动力学控制中的一个组成部分的观点是一致的。
These studies were designed to investigate the relationship between cerebral prostanoid synthesis and pial arterial caliber in chloralose-anesthesized newborn pigs with normal blood gases and pH and during combined arterial hypoxia and hypercapnia. Piglets less than 5 days old were equipped with closed cranial windows to allow direct observation of pial vessels, application of prostaglandin E2, and sampling of cortical subarachnoid cerebrospinal fluid. We found that prostanoids accumulate in cerebrospinal fluid on the cortical surface. The only prostanoid detected in arterial blood was 6-keto-prostaglandin F1.alpha. [442 .+-. 74 pg/ml (radioimmunoassay)]. Only small quantities of 6-keto-prostaglandin F1.alpha. (214 .+-. 53 pg/ml) and thromboxane B2 (122 .+-. 18 pg/ml) were found in cerebrospinal fluid from the cisterna magna. Higher concentrations of 6-keto-prostaglandin F1.alpha. (1056 .+-. 159 pg/ml), thromboxane B2 (229 .+-. 64 pg/ml), and prostaglandin E2 (4235 .+-. 269 pg/ml) were found in cortical subarachnoid fluid. In contrast to arterial and cisternal concentrations, the concentrations of 6-keto-prostaglandin F1.alpha., thromboxane B2, and prostaglandin E2 in cortical subarachnoid fluid were increased reversibly by ventilation with 9% carbon dioxide, 10% oxygen, (6-keto-prostaglandin F1.alpha., 5436 .+-. 1576 pg/ml; thromboxane B2, 694 .+-. 122 pg/ml; and, prostaglandin E2, 12,455 .+-. 3688 pg/ml). Further, pial arteries dilated in response to topical application of prostaglandin E2 at the concentration that was found in cortical subarachnoid fluid during combined hypoxia and hypercapnia. Systemic administration of indomethacin trihydrate (5 mg/kg) markedly reduced cortical subarachnoid fluid prostanoid concentrations and attenuated the pial artery vasodilation induced by combined hypoxia and hypercapnia. Our data are consistent with the hyothesis that cerebral prostanoids represent an integral component in cerebral hemodynamic control in the neonatal pig.