INDUCTION, ISOLATION AND A CHARACTERIZATION OF THE LIPID-CONTENT OF PLASMA VITELLOGENIN FROM 2 SALMO SPECIES - RAINBOW-TROUT (SALMO-GAIRDNERI) AND SEA TROUT (SALMO-TRUTTA)
INDUCTION, ISOLATION AND A CHARACTERIZATION OF THE LIPID-CONTENT OF PLASMA VITELLOGENIN FROM 2 SALMO SPECIES - RAINBOW-TROUT (SALMO-GAIRDNERI) AND SEA TROUT (SALMO-TRUTTA)
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DOI：
10.1016/0305-0491(85)90081-1
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发表时间：
1985-01-01
影响因子：
2.2
通讯作者：
HAUX, C
中科院分区：
文献类型：
--
作者：
NORBERG, B;HAUX, C
雌二醇-17.β 在幼年虹鳟 (S. gairdneri) 和幼年海鳟 (S. trutta) 中诱导卵黄蛋白原合成。描述了通过 MgCl2-EDTA 沉淀和随后在 DEAE-Sephacel 上进行阴离子交换层析从鳟鱼血浆中纯化卵黄原蛋白的程序。纯化的虹鳟鱼和海鳟卵黄原蛋白的总脂质含量分别为 18% 和 19%。大约 2/3 的脂质是磷脂，其余由甘油三酯和胆固醇组成。对脱脂卵黄蛋白原的 P 测定得出虹鳟鱼卵黄蛋白原中的 P 含量为 0.63%，海鳟卵黄蛋白原中的 P 含量为 0.58%。通过 Sepharose 6B 凝胶过滤估算，虹鳟鱼和海鳟鱼的天然（二聚体）卵黄原蛋白的表观分子量均为 440,000。
Vitellogenin synthesis is induced in juvenile rainbow trout (S. gairdneri) and juvenile sea trout (S. trutta) by estradiol-17.beta.. A purification procedure for vitellogenin from trout plasma by precipitation with MgCl2-EDTA and subsequent anion exchange chromatography on DEAE-Sephacel is described. The total lipid contents of purified rainbow trout and sea trout vitellogenins are 18 and 19%, respectively. Approximately 2/3 of the lipids are phospholipids, while the remainder consists of triglycerides and cholesterol. P determinations on delipidated vitellogenin yield a P content of 0.63% in rainbow trout and 0.58% in sea trout vitellogenin. Native (dimeric) vitellogenins from rainbow trout and sea trout both have an apparent MW of 440,000, when estimated by gel filtration on Sepharose 6B.