Metabolomics Reveals Dynamic Metabolic Changes Associated with Age in Early Childhood.
Metabolomics Reveals Dynamic Metabolic Changes Associated with Age in Early Childhood.
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代谢组学揭示了与儿童早期相关的动态代谢变化。
DOI：
10.1371/journal.pone.0149823
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Huang JL
中科院分区：
文献类型：
--
作者：
Chiu CY;Yeh KW;Lin G;Chiang MH;Yang SC;Chao WJ;Yao TC;Tsai MH;Hua MC;Liao SL;Lai SH;Cheng ML;Huang JL
对控制早期生命快速生长的代谢过程仍缺乏详细的了解。这项研究的目的是调查健康儿童在整个童年早期与年龄相关的代谢变化。来自出生队列的健康儿童参加了这项研究，从出生到4岁。用核磁共振氢谱结合主成分分析和偏最小二乘判别分析等多元统计分析方法，对6个月龄和1、2、3、4岁龄大鼠的尿代谢物进行分析。使用MetPA网络工具进行代谢途径分析。共采集30名健康儿童的105份尿样进行分析。使用有监督的偏最小二乘法(PLS-DA)识别有助于年龄组区分的代谢物(Q2=0.60；R2=0.66)。6个月大的儿童尿三甲胺氮氧化物(TMAO)和甜菜碱水平显著升高。尿甘氨酸和谷氨酰胺水平在6个月龄后显著下降，尿肌酸和肌酐也随之代偿性增加。使用MetPA的代谢途径分析显示，在所有被评估的年龄段中，与氮代谢相关的能量产生相似。与氨基酸代谢相关的途径在6个月和1岁的婴儿中显著不同，而与碳水化合物代谢相关的途径在2岁和3岁的儿童中显著不同。尿液代谢组学理想地代表了不同年龄段的动态代谢变化。尿液代谢特征在婴儿出生后的第一年内会发生显著变化，这可能会提供有关婴儿营养和生长发育的重要信息。
A detailed understanding of the metabolic processes governing rapid growth in early life is still lacking. The aim of this study was to investigate the age-related metabolic changes in healthy children throughout early childhood. Healthy children from a birth cohort were enrolled in this study from birth through 4 years of age. Urinary metabolites were assessed at 6 months, and 1, 2, 3, and 4 yr of age by using 1H-nuclear magnetic resonance (NMR) spectroscopy coupled with multivariate statistical analysis including principal components analysis (PCA) and partial least-squares discriminant analysis (PLS-DA). Metabolic pathway analysis was performed using the MetPA web tool. A total of 105 urine samples from 30 healthy children were collected and analyzed. Metabolites contributing to the discrimination between age groups were identified by using supervised PLS-DA (Q2 = 0.60; R2 = 0.66). A significantly higher urinary trimethylamine N-oxide (TMAO) and betaine level was found in children aged 6 months. Urinary glycine and glutamine levels declined significantly after 6 months of age and there was a concomitant compensatory increase in urinary creatine and creatinine. Metabolic pathway analysis using MetPA revealed similar nitrogen metabolism associated energy production across all ages assessed. Pathways associated with amino acid metabolism were significantly different between infants aged 6 months and 1 year, whereas pathways associated with carbohydrate metabolism were significantly different between children at ages 2 and 3 years. Urine metabolomics ideally represents dynamic metabolic changes across age. Urinary metabolic profiles change significantly within the first year of life, which can potentially provide crucial information about infant nutrition and growth.