Use of an adaptation of a commercially available PCR assay aimed at diagnosis of chlamydia and gonorrhea to detect Trichomona vaginalis in urogenital specimens
Use of an adaptation of a commercially available PCR assay aimed at diagnosis of chlamydia and gonorrhea to detect Trichomona vaginalis in urogenital specimens
复制标题
DOI：
10.1128/jcm.44.2.366-373.2006
复制
发表时间：
2006-02-01
影响因子：
9.4
通讯作者：
Williams, JA
中科院分区：
文献类型：
--
作者：
Van Der Pol, B;Kraft, CS;Williams, JA
利用市售沙眼衣原体和淋病奈瑟菌检测试剂，对阴道毛滴虫PCR检测在性传播疾病诊所就诊的男女中的感染情况进行了评估。评估包括三组引物，宫颈拭子，阴道拭子和尿液，以及各种储存条件。TVK3/TVK7引物组灵敏度在69.5 ~ 96.8%之间，是我们手上的最佳引物。在所有的比较中，阴道生殖道绦虫PCR优于常规显微镜诊断（女性）和培养（男性）（P < 0.05）。该检测方法对所有类型的样品均有效，阴道拭子在室温下可稳定达7天。使用沙眼衣原体制备的样品和试剂。淋病PCR检测允许将阴道滴虫PCR诊断纳入常规临床检测。
Trichomonas vaginalis PCR using reagents from a commercially available assay for Chlamydia trachomatis and Neisseria gonorrhoeae was evaluated for detection of infection in women and men attending a sexually transmitted disease clinic. Evaluations included three primer sets, endocervical swabs, vaginal swabs and urine, and various storage conditions. The TVK3/TVK7 primer set was optimal in our hands with sensitivities ranging from 69.5 to 96.8%. In all comparisons, T. vaginalis PCR performed better than routine diagnostics using microscopy for women and culture for men (P > 0.05). The assay performed well for all sample types tested, and vaginal swabs were stable for up to 7 days at ambient temperature. Using samples prepared for, and reagents from, the C. trachomatis-N. gonorrhoeae PCR assay allowed incorporation of T. vaginalis PCR diagnosis into routine clinical testing.