Genetic inheritance of dinitroaniline resistance in an annual ryegrass population
Genetic inheritance of dinitroaniline resistance in an annual ryegrass population
复制标题
一年生黑麦草群体对二硝基苯胺抗性的遗传研究
DOI：
10.1016/j.plantsci.2019.02.019
复制
发表时间：
2019-06-01
期刊：
影响因子：
5.2
通讯作者：
Powles, Stephen
中科院分区：
文献类型：
--
作者：
Chen, Jinyi;Lu, Huan;Powles, Stephen
越来越多的杂草对二硝基苯胺类除草剂产生抗性，这就需要研究影响杂草抗性进化的进化因素，包括抗性性状的遗传。在这项研究中，我们调查了氟乐灵抗性的遗传控制在一个良好的特点硬直黑麦草高德。来自西澳大利亚州的人口。将该群体纯化以包含对于瓦尔-202-Phe α-微管蛋白突变纯合的植物，并用作抗性（R）亲本并与敏感（S）亲本杂交以产生8个相互的F1家系。8个F1家系的氟乐灵剂量反应曲线表明，该群体的氟乐灵抗性是以不完全隐性核性状遗传的。将F1植物在每个家系内杂交以建立8个假F2（psi-F2）家系。用氟乐灵120和480 g a. i.的区分率测定了psi-F2家系的抗性和感病分离比。ha（-1）在480 g a.i. ha ~（-1）氟乐灵的分离比，除一个外，几乎所有的psi-F_2家系均符合1：3（抗：感）的单隐性基因控制模型。然而，在120 g a. i. ha（-1）氟乐灵，半数家系的分离率不符合该模型，表明一个或多个基因参与抗性，但分离率较低。这些结果表明，该菌对氟乐灵抗性遗传的复杂性。具有瓦尔-202-Phe α-微管蛋白突变的刚性群体。
The increasing number of weedy species resistant to dinitroaniline herbicides warrants studies on the evolutionary factors contributing to resistance evolution, including genetic inheritance of resistance traits. In this study, we investigated the genetic control of trifluralin resistance in a well-characterised Lolium rigidum Gaud. population from Western Australia. This population was purified to contain plants homozygous for the Val-202-Phe alpha-tubulin mutation, and used as the resistant (R) parents and crossed with susceptible (S) parents to produce eight reciprocal F1 families. Trifluralin dose response curves of the eight Fl families indicate that trifluralin resistance in this population is inherited as an incomplete recessive nuclear trait. The Fl plants were crossed within each families to establish eight pseudo-F2 (psi-F2) families. Segregation ratio of resistance and susceptibility in psi-F2 families were determined using the discriminating trifluralin rates of 120 and 480 g a.i. ha(-1)At 480 g a.i. ha(-1 )trifluralin, the segregation ratio in almost all psi-F2 families (except one) was fit to 1:3 (resistance: susceptibility) one recessive gene control model. However, at 120 g a.i. ha(-1) trifluralin, the segregation ratios in half of the families did not fit this model, indicating involvement of one or more genes in resistance at the lower rate. These results showed complexity of genetic inheritance of trifluralin resistance in this L. rigidum population possessing the Val-202-Phe alpha-tubulin mutation.