Enteric and nasal shedding of bovine torovirus (Breda virus) in feedlot cattle
Enteric and nasal shedding of bovine torovirus (Breda virus) in feedlot cattle
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DOI：
10.2460/ajvr.2002.63.342
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发表时间：
2002-03-01
影响因子：
1
通讯作者：
Saif, LJ
中科院分区：
文献类型：
--
作者：
Hoet, AE;Cho, KO;Saif, LJ
牛-为了评估粪便和鼻腔脱落模式牛圆环病毒（BoTV）在牛在抵达时，并定期在整个第一个21天后抵达饲养场。动物-57阉牛。程序-粪便和鼻拭子样本收集0，4，14，和21天后抵达进行测试BoTV，使用ELISA。使用逆转录酶-聚合酶链反应（RT-PCR）分析了BoTV检测阳性和阴性小牛的样本子集。配对的血清样品收集在第0和21天，并测试BoTV抗体，使用血凝抑制assay. Results粪便脱落的BoTV的总体率为21 57（37%）通过ELISA和40 42（95%）通过RT-PCR与峰值脱落在第4天。腹泻在脱落BoTV的小牛中比未脱落病毒的小牛更常见（比值比，1.72）。BoTV的鼻脱落的总体率通过ELISA为57例中的15例（26%），通过RT-PCR为42例中的42例（100%），在第0天达到峰值脱落。RT-PCR产物的特异性通过序列分析证实。大约93%的小牛血清转化为BoTV（滴度增加> 4倍）在疾病和治疗方面，未检测到脱落BoTV的小牛和非脱落小牛之间存在差异，这可能是因为研究中的牛数量较少。结论和临床相关性-本研究证实了饲养场牛中的BoTV感染，包括鼻分泌物中的BoTV抗原和病毒RNA，以及在到达饲养场后的前21天期间的脱落模式。
Objective-To assess fecal and nasal shedding patterns of bovine torovirus (BoTV) in cattle at time of arrival and periodically throughout the first 21 days after arrival at a feedlot.Animals-57 steers.Procedure-Fecal and nasal-swab samples collected on days 0, 4, 14, and 21 after arrival were tested for BoTV, using ELISA. A subset of samples from calves testing positive and negative for BoTV was analyzed, using reverse transcriptase-polymerase chain reaction (RT-PCR). Paired serum samples were collected on days 0 and 21 and tested for BoTV antibodies, using a hemagglutination inhibition assay.Results-Overall rate of fecal shedding of BoTV was 21 of 57 (37%) by ELISA and 40 of 42 (95%) by RT-PCR with peak shedding on day 4. Diarrhea was more common in calves shedding BoTV than those not shedding the virus (odds ratio, 1.72). Overall rate of nasal shedding of BoTV was 15 of 57 (26%) by ELISA and 42 of 42 (100%) by RT-PCR, with peak shedding on day 0. Specificity of the RT-PCR product was confirmed by sequence analysis. Approximately 93% of the calves seroconverted to BoTV (> 4-fold increase in titer) Differences were not detected between calves shedding BoTV and nonshedders in relation to disease and treatments, perhaps because of the low number of cattle in the study.Conclusions and Clinical Relevance-This study confirmed BoTV infections in feedlot cattle, including BoTV antigen and viral RNA in nasal secretions, and the shedding pattern during the first 21 days after arrival in a feedlot.