Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers
Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers
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单分子磁镊表征人类端粒 G-四链体之间的相互作用
DOI：
10.1088/1674-1056/27/6/068701
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发表时间：
2018
影响因子：
1.7
通讯作者：
Li Wei
中科院分区：
文献类型：
--
作者：
Wang Yi-Zhou;Hou Xi-Miao;Ju Hai-Peng;Xiao Xue;Xi Xu-Guang;Dou Shuo-Xing;Wang Peng-Ye;Li Wei
端粒G-四链体在调节基因组稳定性中起着重要作用。尽管对单体G-四链体的结构和动力学进行了广泛的研究，但G-四链体之间的相互作用仍存在争议。在这项工作中，我们采用磁镊研究的折叠和展开动力学的两个连续的G-四链体在100 mM的K+缓冲液。揭示了G-四链体之间的相互作用及其对G-四链体动力学的影响。进一步发现G-四链体之间的接头序列在两个G-四链体之间的相互作用中起重要作用。我们的研究结果提供了一个高分辨率的洞察动力学的多聚体G-四链体和基因组的稳定性。
Human telomeric G-quadruplex plays a crucial role in regulating the genome stability. Despite extensive studies on structures and kinetics of monomeric G-quadruplex, the interaction between G-quadruplexes is still in debate. In this work, we employ magnetic tweezers to investigate the folding and unfolding kinetics of two contiguous G-quadruplexes in 100-mM K+ buffer. The interaction between G-quadruplexes and the consequent effect on the kinetics of G-quadruplex are revealed. The linker sequence between G-quadruplexes is further found to play an important role in the interaction between two G-quadruplexes. Our results provide a high-resolution insight into kinetics of multimeric G-quadruplexes and genome stability.