Expression of connexin30.2 in interneurons of the central nervous system in the mouse
Expression of connexin30.2 in interneurons of the central nervous system in the mouse
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DOI：
10.1016/j.mcn.2007.09.003
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发表时间：
2008-01-01
影响因子：
3.5
通讯作者：
Willecke, Klaus
中科院分区：
文献类型：
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作者：
Kreuzberg, Maria M.;Deuchars, Jim;Willecke, Klaus
电突触，特别是由连接蛋白（Cx）36组成的间隙连接，已被认为是同步神经元网络振荡。最近，我们产生了Cx30.2缺陷小鼠，其在Cx30.2基因调控元件的控制下表达β-半乳糖苷酶。在中枢神经系统中，代表Cx30.2表达的β-半乳糖苷酶活性仅限于NeuN阳性细胞，因此将Cx30.2鉴定为新的神经元连接蛋白。在海马中，共免疫荧光分析显示β-半乳糖苷酶/Cx30.2在GABA能抑制性中间神经元中表达，如小白蛋白和生长抑素阳性篮状细胞、轴-轴细胞、双层细胞或东方腔隙-分子细胞。类似于94%的表达Cx30.2的小清蛋白阳性中间神经元也表达Cx 36。通过记录海马脑片的场电位，我们发现Cx30.2（+/+）和Cx30.2（LacZ/LacZ）小鼠的基础兴奋和兴奋-抑制平衡没有差异，而且这些动物的间隙连接依赖性γ振荡和波纹振荡的频率和功率也相似。这表明，Cx30.2在中间神经元中的缺乏可以在很大程度上由其他连接蛋白（最有可能是Cx 36）补偿。(C)2007年爱思唯尔公司All rights reserved.
Electrical synapses, particularly gap junctions composed of connexin (Cx) 36, have been suggested to synchronize neuronal network oscillations. Recently, we generated Cx30.2-deficient mice which express beta-galactosidase under control of Cx30.2 gene regulatory elements. In the central nervous system beta-galactosidase activity representing Cx30.2 expression was restricted to NeuN-positive cells, thus identifying Cx30.2 as new neuronal connexin. In the hippocampus, co-immunofluorescence analyses revealed beta-galactosidase/Cx30.2 expression in GABAergic inhibitory interneurons such as parvalbumin- and somatostatin-positive basket, axo-axonic, bistratified or oriens lacunosum-moleculare cells. similar to 94% of the Cx30.2 expressing parvalbumin-positive interneurons also expressed Cx36. Performing field potential recordings from hippocampal slices we found no differences in basal excitation and excitation-inhibition balance between Cx30.2(+/+) and Cx30.2(LacZ/LacZ) mice.Furthermore, frequency and power of gap junction dependent gamma and ripples oscillations were similar in these animals. This suggests that the lack of Cx30.2 in interneurons can be largely compensated by other connexins, most likely Cx36. (C) 2007 Elsevier Inc. All rights reserved.