tLyP-1-conjugated mesoporous silica nanoparticles for tumor targeting and penetrating hydrophobic drug delivery
tLyP-1-conjugated mesoporous silica nanoparticles for tumor targeting and penetrating hydrophobic drug delivery
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DOI：
10.1007/s11051-013-2105-4
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发表时间：
2013-11
影响因子：
2.5
通讯作者：
Baiyao Xu;Y. Ju;Guanbin Song;Yanbin Cui
中科院分区：
文献类型：
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作者：
Baiyao Xu;Y. Ju;Guanbin Song;Yanbin Cui
介孔二氧化硅纳米颗粒（MSNs）是最具吸引力的靶向药物递送候选材料之一，在这一过程中，纳米颗粒必须以高速和高效的方式内化到靶向细胞中。因此，有必要将靶向配体偶联到纳米载体表面，以触发受体介导的快速内吞作用和有效的细胞摄取，这是在识别和选择性结合靶细胞膜受体后发生的。本文描述了一种基于msn（约100 nm大小）的肿瘤靶向穿透给药系统（DDS）。通过肿瘤归巢和穿透肽tlyp1的移植使msn功能化。负载喜树碱（CPT）的MSN-tLyP-1对HeLa细胞和MCF-7细胞具有很强的靶向和穿透作用，并能诱导这些细胞死亡。此外，CPT对人间充质干细胞（hMSCs）的不良副作用被最小化，因为纳米颗粒选择性地靶向肿瘤细胞，并且几乎没有疏水性CPT释放到培养基或血液中。结果表明，MSN-tLyP-1 DDS在靶向肿瘤的疏水抗癌药物递送方面具有很大的潜力。
Mesoporous silica nanoparticles (MSNs) are among the most appealing candidates for targeted drug delivery, a process for which it is essential that nanoparticles be internalized into targeted cells with high speed and efficiency. Therefore, it is necessary to conjugate a targeting ligand to the surface of a nanocarrier in order to trigger rapid receptor-mediated endocytosis and effective cellular uptake, which occurs following recognition and selective binding to a target cell’s membrane receptor. Here, a tumor targeting and penetrating drug delivery system (DDS) based on MSNs (~100 nm in size) is described. The MSNs were functionalized by engrafting with the tumor-homing and penetrating peptide tLyP-1. The fabricated MSN–tLyP-1 loaded with camptothecin (CPT) showed a robust targeting and penetrating efficiency to HeLa cells and MCF-7 cells and induced the death of these cells. Moreover, the adverse side effect of CPT on human mesenchymal stem cells (hMSCs) was minimized, because the nanoparticles were selectively targeted to the tumor cells, and little hydrophobic CPT was released into the culture medium or blood. The results indicate that the MSN–tLyP-1 DDS has great potential for the delivery of hydrophobic anticancer drugs to target tumors.