Evaluation of the Selected 12-locus MIRU for Genotyping Beijing Family Mycobacterium Tuberculosis in Korea
Evaluation of the Selected 12-locus MIRU for Genotyping Beijing Family Mycobacterium Tuberculosis in Korea
复制标题
DOI：
10.4046/trd.2009.67.6.499
复制
发表时间：
2009-12-01
影响因子：
2.9
通讯作者：
Lew, Woojin
中科院分区：
文献类型：
--
作者：
Kang, Heeyoon;Ryoo, Sungweon;Lew, Woojin
背景：分枝杆菌散布重复单位（MIRU）主要位于分散在结核分枝杆菌基因组中的基因间区域。选定的 MIRU 基因座由 12 个基因座组组成，表现出对从韩国江原道收集的结核分枝杆菌分离株的高辨别力。为了评估其区分结核分枝杆菌菌株的能力，使用 IS6110 RFLP 和 MIRU 方法对 45 个临床分离株进行了基因分型。 方法：所有样本均从 2007 年 1 月至 2007 年 12 月期间从韩国江原道公共卫生中心登记的居民结核病患者中采集。从临床分离的分枝杆菌菌株中提取了总共 45 个 DNA，并使用 IS6110 RFLP（MIRU 方法）进行了基因分型。结果：我们在 45 个样本中比较了 12-MIRU 与 IS6110 RFLP，12 位点版本的区分能力较低（Hunter-Gaston 区分指数 [HGDI]：0.959 vs 0.998； 57.78% 的聚集性病例 vs 8.89%)。结论：当其他基因座的其他组合用于对北京家族菌株占主导地位的韩国结核分枝杆菌进行基因分型时，此 12 位点 MIRU 可能很有用。
Background: Mycobacterial Interspersed Repetitive Units (MIRUs) that are located mainly in intergenic regions dispersed throughout the Mycobacterium tuberculosis genome. The selected MIRU loci, which were composed of a 12- locus set, demonstrated a high power for discrimination of Mycobacterium tuberculosis isolates collected from Kangwon province of Korea. To evaluate its ability to discriminate the M. tuberculosis strains, 45 clinical isolates were genotyped using the methods IS6110 RFLP and MIRU.Methods: All the samples were collected during the period from January 2007 to December 2007 from TB patients, who were residents and registered to a public health center of Kangwon Province in Korea. A total of 45 DNAs were extracted from clinical isolated mycobacterial strains and genotyped using IS6110 RFLP, the MIRU method.Results: We compared the 12-MIRU with IS6110 RFLP in the 45 samples, the 12-locus version offered less discriminatory power (Hunter-Gaston discriminatory index [HGDI]: 0.959 vs 0.998; 57.78% of clustered cases vs 8.89%).Conclusion: This 12-locus MIRU can be useful when additional combinations of other loci for genotyping M. tuberculosis in Korea where the Beijing family strains are dominant.