Divergent expression patterns of Sox9 duplicates in teleosts indicate a lineage specific subfunctionalization
Divergent expression patterns of Sox9 duplicates in teleosts indicate a lineage specific subfunctionalization
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DOI：
10.1007/s00427-005-0477-x
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发表时间：
2005-06-01
影响因子：
2.4
通讯作者：
Schartl, M
中科院分区：
文献类型：
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作者：
Klüver, N;Kondo, M;Schartl, M
SRY相关的HMG-box基因是几个发育过程的关键调节基因。SOX9编码哺乳动物软骨形成和睾丸确定所需的转录因子。在斑马鱼(Danio Rerio)和刺鱼(Gasterbone Aculeatus)中，存在两个Sox9的同源基因。到目前为止，在青竹中只发现了一种Sox9。我们现在已经分离出第二个Sox9基因。序列分析、系统发育分析、连锁图谱和表达谱分析表明，青冈Sox9a和Sox9b是共同源的。在胚胎发育过程中，Sox9a和Sox9b在颅面软骨成分中的表达模式是不同的，但有相当大的重叠。将斑马鱼Sox9a和Sox9b的表达模式与青蛙Sox9a和Sox9b的表达结构域进行比较，发现有些相同，但另一些明显不同。我们得出结论，在硬骨鱼分化之前，Sox9调节子功能没有被分割，并进化到谱系特异的表达结构域。
Sry-related HMG-box genes are key regulators of several developmental processes. Sox9 encodes a transcription factor required for cartilage formation and testis determination in mammals. In zebrafish (Danio rerio) and stickleback (Gasterosteus aculeatus) two co-orthologs of Sox9 are present. To date, only one Sox9 had been identified in medaka (Oryzias latipes). We have now isolated the second Sox9 gene. Sequence analysis, phylogenetic data, linkage mapping as well as expression pattern all together suggest that the medaka Sox9a and Sox9b are co-orthologs. During embryogenesis, the expression pattern of Sox9a and Sox9b are distinct but overlap considerably in craniofacial cartilage elements. Comparing the zebrafish Sox9a and Sox9b expression patterns with medaka Sox9a and Sox9b expression domains revealed that some are identical but others are clearly different. We conclude that Sox9 regulatory subfunctions were not partitioned before divergence of the teleosts and evolved to lineage-specific expression domains.