THE DEVELOPMENT OF PLASMID-FREE STRAINS OF AGROBACTERIUM-TUMEFACIENS BY USING INCOMPATIBILITY WITH A RHIZOBIUM-MELILOTI PLASMID TO ELIMINATE PATC58
THE DEVELOPMENT OF PLASMID-FREE STRAINS OF AGROBACTERIUM-TUMEFACIENS BY USING INCOMPATIBILITY WITH A RHIZOBIUM-MELILOTI PLASMID TO ELIMINATE PATC58
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DOI：
10.1016/0147-619x(85)90062-9
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发表时间：
1985-01-01
期刊：
影响因子：
2.6
通讯作者：
PUHLER, A
中科院分区：
文献类型：
--
作者：
HYNES, MF;SIMON, R;PUHLER, A
A.通过导入根瘤菌R.苜蓿属植物质粒pRme 41 a与pAtC 58不相容。随后通过在超最适温度（40 ℃）下生长消除LBA 275的驻留Ti质粒pRme 41 a和pTiC 58。C）。由此产生的无质粒农杆菌菌株，UBAPF 1和UBAPF 2，证明是非常有用的根瘤菌质粒的研究。隐藏质粒pAtC 58的丢失对农杆菌菌株的肿瘤形成能力没有影响;当Ti质粒存在时，在Kalpene daigremontiana上形成正常肿瘤。
A. tumefaciens strains LBA275 and LBA290 were cured of their cryptic plasmid pAtC58 by the introduction of the R. meliloti plasmid pRme41a, which is incompatible with pAtC58. pRme41a and pTiC58, the resident Ti plasmid of LBA275, were subsequently eliminated by growth at supraoptimal temperature (40.degree. C). The resulting plasmid-free Agrobacterium strains, UBAPF1 and UBAPF2, proved extremely useful for the study of Rhizobium plasmids. The loss of the cryptic plasmid pAtC58 has no effect on the tumor-forming ability of the Agrobacterium strains; when the Ti plasmid is present, normal tumors are formed on Kalanchoe daigremontiana.