Radioimmunodetection of cancer with radiolabeled antibodies to alpha-fetoprotein.
Radioimmunodetection of cancer with radiolabeled antibodies to alpha-fetoprotein.
复制标题
使用放射性标记的甲胎蛋白抗体对癌症进行放射免疫检测。
作者：
E. Kim;F. Deland;Nelson Mo;S. Bennett;G. Simmons;E. Alpert;D. Goldenberg
应用131I标记的羊抗甲胎蛋白(AFP)抗体，对16例经组织学证实的恶性肿瘤患者进行了放射免疫检测。胸部和腹部的图像是用闪烁相机拍摄的，通常是在注射1-2.5mCI的放射性碘化抗体后24和48小时。使用计算机辅助处理减去99mTc本底放射性，以增强免疫核素显示的肿瘤的检测和定位。放射免疫检测可以显示5个AFP产生的肿瘤所涉及的所有12个部位，而131I标记的正常山羊免疫球蛋白G在1例放射性AFP抗体达到肿瘤放射定位的患者中未能显示类似的结果。在5名肿瘤不会产生AFP的患者中，16个肿瘤部位中的6个也进行了免疫核素扫描，显示了他们的大肿瘤。AFP阳性和AFP缺陷型肿瘤的131I图像平均计数密度比分别为3.20和1.96。对于含有AFP的肿瘤，图像对比度明显更大，减影技术将图像对比度提高了2倍以上。根据这些初步结果，该方法的敏感度(真阳性率)为100%，特异度(真阴性率)为80%，准确率为85%。这项研究表明，用放射性AFP抗体对癌症进行放射免疫检测在评估含AFP的肿瘤患者中是有用的。
Sixteen patients with histologically proven malignant neoplasia were investigated by radioimmunodetection, using goat anti-alpha-fetoprotein (AFP) antibody radiolabeled with 131I. Images of the chest and abdomen were made with a scintillation camera, usually at 24 and 48 hr following injection of 1 to 2.5 mCi of radioiodinated antibody. Computer-assisted processing for the subtraction of 99mTc background radioactivity was used to enhance the detection and localization of tumors visualized by immune scintigraphy. All 12 sites involved by five AFP-producing tumors could be demonstrated by radioimmunodetection, while normal goat immunoglobulin G labeled with 131I failed to show similar results in one of the patients in whom the radioactive AFP antibody achieved tumor radiolocalization. Five patients in whom the tumors were not expected to produce AFP also had their large tumors demonstrated by immune scintigraphy in 6 of 16 tumor sites. The average tumor to non-tumor 131I image count density ratios were 3.20 and 1.96 for the AFP-containing and putatively AFP-deficient tumors, respectively. The image contrast was significantly greater for the AFP-containing tumors, and the subtraction technique enhanced the image contrast more than 2-fold. Based upon these initial results, the sensitivity of the method (true-positive rate) was 100%, its specificity (true-negative rate) was 80%, and the accuracy of the technique was 85%. This study thus indicates that radioimmunodetection of cancer with radioactive AFP antibodies can be useful in the evaluation of patients with AFP-containing neoplasms.