Dietary n-3 polyunsaturated fatty acids alter the expression of genes involved in prostaglandin biosynthesis in the bovine uterus
Dietary n-3 polyunsaturated fatty acids alter the expression of genes involved in prostaglandin biosynthesis in the bovine uterus
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DOI：
10.1016/j.theriogenology.2008.05.048
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发表时间：
2008-09-15
期刊：
影响因子：
2.8
通讯作者：
Waters, S. M.
中科院分区：
文献类型：
--
作者：
Coyne, G. S.;Kenny, D. A.;Waters, S. M.
营养在奶牛繁殖力的调节中起着至关重要的作用。越来越多的证据表明，饮食中的长链n-3多不饱和脂肪酸(LCn-3 PUFA)可能是某些生殖过程的特异性调节因子。体外研究表明，n-3多不饱和脂肪酸、二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)可能通过抑制子宫前列腺素F-2α(PGF(2α))的合成而发挥关键作用，PGF(2α)是控制牛发情周期和早期胚胎存活的核心因素。本研究的目的是确定饮食中n-3多不饱和脂肪酸对子宫内膜调控PGF(2α)生物合成关键基因mRNA表达的影响。用低(Con；It=10)或高(高PUFA；It=10)n-3PUFA饲喂小母牛45d，屠宰后取子宫内膜组织。分析后，根据其多不饱和脂肪酸浓度对每个饮食组中的组织进行排序，并分别使用高PUFA和高CON中最高(It=7)和最低(It=7)的组织进行基因表达研究。高PUFA组子宫内膜n-3PUFA浓度是CON组的两倍多(P<；0.05)，EPA浓度是CON组的七倍多(P<；0.01)。高PUFA组子宫内膜花生四烯酸浓度低于CON组(P<；0.001)。从所有子宫内膜组织中提取总RNA，进行实时逆转录聚合酶链式反应(RT-PCR)，比较11个已知参与子宫生物合成的基因的相对表达。高PUFA内膜组织中前列腺素E合成酶(PGES)、PPARα和PPAR 8的mRNA表达增加(P<0.05)，而磷脂酶A(2)(PLA2)的mRNA表达降低(P=0.06)。PUFA和CON组织中催产素受体(OTR)、磷脂酶C(PLC)、环氧合酶-1(COX-1)、环氧合酶-2(COX-2)、前列腺素E(2)-9-酮还原酶(9-KPR)、前列腺素F合成酶(PGFS)和核转录因子PPAR-γ等基因的表达差异无统计学意义(P>；0.05)。总之，这些结果表明，控制子宫PGF(2α)生物合成的关键基因可以通过饮食中的Lcn-3多不饱和脂肪酸来调节，这可能会影响子宫功能和胚胎存活。(C)2008 Elsevier Inc.保留所有权利。
Nutrition plays a critical role in the regulation of cow fertility. There is emerging evidence that dietary long chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) may act as specific regulators of some reproductive processes. In vitro studies suggest that the n-3 PUFAs, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) may play pivotal roles by suppressing the synthesis of uterine prostaglandin F-2 alpha(PGF(2 alpha)) which is centrally involved in the control of the bovine oestrous cycle and in early embryo survival. The objective of the current study was to determine the effect of dietary inclusion of n-3 PUFA on uterine endometrial mRNA expression of key genes regulating PGF(2 alpha) biosynthesis. Beef heifers were fed either a low (CON; it = 10) or high (HIGH PUFA; it = 10) n-3 PUFA diet for 45 days and endometrial tissues were harvested following slaughter. Following analysis, tissues within each dietary group were ranked on the basis of their PUFA concentrations and the highest (it = 7) and lowest (it = 7) within each of HIGH PUFA and CON, respectively, were used in gene expression studies. Endometrial n-3 PUFA concentrations were more than two-fold higher (P < 0.05) and EPA concentrations alone more than seven-fold higher (P < 0.01) in the HIGH PUFA than the CON group. Endometrial concentrations of arachidonic acid, were lower (P < 0.001) in the tissues from HIGH PUFA than those from the CON group. Total RNA was isolated from all endometrial tissues and real-time reverse transcription (RT) PCR conducted to compare the relative expression of 11 genes with known involvement in uterine biosynthesis of 2-series prostaglandins. Expression of mRNA for prostaglandin E synthase (PGES) and peroxisome proliferator-activated receptors, PPAR a and 8 was increased (P < 0.05) while mRNA expression of phospholipase A(2) (PLA(2)) was decreased (P = 0.06) in the HIGH PUFA endometrial tissues. Expression of genes coding for the oxytocin receptor (OTR), phospholipase C (PLC), cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), PGE(2) 9-ketoreductase (9-KPR), prostaglandin F synthase (PGFS), and the nuclear transcription factor, PPAR gamma was not different (P > 0.05) between HIGH PUFA and CON tissues. Overall the results indicate that key genes regulating uterine PGF(2 alpha) biosynthesis can be regulated by dietary inclusion of LC n-3 PUFA which may influence uterine function and embryo survival. (c) 2008 Elsevier Inc. All rights reserved.