Methanomicrobium antiquum sp. nov., a hydrogenotrophic methanogen isolated from deep sedimentary aquifers in a natural gas field
Methanomicrobium antiquum sp. nov., a hydrogenotrophic methanogen isolated from deep sedimentary aquifers in a natural gas field
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古董甲烷微生物 sp.
DOI：
10.1099/ijsem.0.001444
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Sakata S and Kamagata Y
中科院分区：
文献类型：
--
作者：
Mochimaru H;Tamaki H;Katayama T;Imachi H;Sakata S and Kamagata Y
一个嗜中温，氢营养型产甲烷菌，指定菌株MobHT，分离自来自深沉积，天然气含水层在日本的沉积物。菌株MobHT利用H2/CO2或甲酸盐进行生长和甲烷产生，但不利用乙醇、1-丙醇、2-丙醇、2-丁醇或环戊醇。此外，乙酸盐和钨是生长所必需的。酵母提取物刺激生长，但不是必需的。细胞运动能力弱，具多鞭毛，呈弯曲杆状（0.8×2.0 µm），单个或成对出现。菌株MobHT在15-40 °C（最适35 °C）和pH 5.9-7.9（最适pH 7.0-7.5）下生长。氯化钠生长范围为0- 5.8%（最佳2%）。基因组DNA的G+C含量为37.6mol%。在基于16 S rRNA基因序列构建的系统发育树中，MobHT菌株与Methanomicrobium移动的（95.4%）聚在一起，与Methanolacinia petroleariaSEBR 4847 T（95.6%）和Methanolacinia paynteriG-2000 T（95.4%）形成一个独立的分支。Methanomicrobium属中的两个种均能利用2-丙醇，而MobHT菌株和Methanomicrobium属中唯一的种--移动的Methanomicrobium则不能。基于表型和系统发育特征，我们提出了一个新的物种的名称，Methanomicrobiumantiquumsp。模式菌株为MobHT（=DSM 21220 T =NBRC 104160 T）。
A mesophilic, hydrogenotrophic methanogen, designated strain MobHT, was isolated from sediments derived from deep sedimentary, natural-gas-bearing aquifers in Japan. Strain MobHTutilized H2/CO2or formate, but not ethanol, 1-propanol, 2-propanol, 2-butanol or cyclopentanol, for growth and methane production. In addition, acetate and tungsten were required for growth. Yeast extract stimulated the growth, but was not required. The cells were weakly motile with multiple flagella, presented as a curved-rod-shaped (0.8×2.0 µm) and occurred singly or in pairs. Strain MobHTgrew at 15–40 °C (optimum 35 °C) and at pH 5.9–7.9 (optimum pH 7.0–7.5). The sodium chloride range for growth was 0–5.8 % (optimum 2 %). The G+C content of the genomic DNA was 37.6 mol%. In the phylogenetic tree based on the 16S rRNA gene sequences, strain MobHTclustered together withMethanomicrobium mobile(95.4 % in sequence similarity), and formed a distinct clade fromMethanolacinia petroleariaSEBR 4847T(95.6 %) andMethanolacinia paynteriG-2000T(95.4 %). The two species of the genusMethanolaciniautilized 2-propanol, whereas strain MobHTandMethanomicrobium mobile, the sole species of the genusMethanomicrobium, do not. Based on phenotypic and phylogenetic features, we propose a novel species for the isolate with the name,Methanomicrobiumantiquumsp. nov. The type strain is MobHT(=DSM 21220T=NBRC 104160T).