Localization of collagen types in regional segments of the fetal bovine aorta.
Localization of collagen types in regional segments of the fetal bovine aorta.
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发表时间：
1989-11
影响因子：
5
通讯作者：
Howard Ps;Macarak Ej
中科院分区：
文献类型：
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作者：
Howard Ps;Macarak Ej
I型，III型和IV型胶原的免疫细胞化学定位在肌和小动脉的胎牛组织和主动脉的不同区域段。发现这些胶原的分布是独特的，不仅在胶原类型和纤维取向方面，而且在被检查的血管的特定区域方面。I型、III型和IV型胶原通常共同分布在血管的内膜和中膜区域，而I型和III型胶原在外膜层中具有不同形态的纤维中显示出选择性关联。检查主动脉的特定节段（近端胸与远端腹），以确定胶原的相对分布和形态学表现。在心脏近端主动脉的升区，I型、III型和IV型胶原共定位于内膜层和中层。I型和III型胶原蛋白在相对较小的外膜层中占主导地位，而IV型胶原蛋白仅与该层中的小血管和毛细血管的介质相关。在主动脉弓远端的胸降区，I型和IV型胶原分布在整个内膜和中层，而III型胶原定位根据用于检测的抗体而变化。外膜主要针对III型胶原染色，其明显组织在折叠的纤维阵列中。这些相同的纤维用I型胶原抗体染色不太强烈。髂总动脉附近的腹主动脉以与所检查器官和组织的肌动脉相似的方式染色，即，I型和IV型胶原蛋白存在于内膜和中层中，而III型胶原蛋白大量定位于外膜层，内膜和中膜的染色差异取决于所使用的抗体。用I型和IV型胶原蛋白抗体对腹主动脉外膜区域进行的额外染色与近端和下降区相同。由于腹主动脉与升胸主动脉相比维持降低的脉动扩张水平，III型胶原分布及其与其他基质组分的相关性在调节血管壁扩张性的范围中可能是重要的。
Types I, III, and IV collagens were localized immunocytochemically in muscular and small arteries of fetal bovine tissues and in different regional segments of the aorta. The distribution of these collagens was found to be unique, not only with respect to collagen type and fiber orientation but also to the specific region of the vessel being examined. Collagen types I, III, and IV often codistribute in the intimal and medial regions of blood vessels, whereas types I and III show selective association in fibers with varied morphology in the adventitial layer. Specific segments of the aorta (proximal thoracic versus distal abdominal) were examined to determine the relative distribution and morphologic presentation of the collagens. In the ascending region of the aorta proximal to the heart, the types I, III, and IV collagen colocalized in the intimal and medial layers. Types I and III collagen predominated in the relatively small adventitial layer, whereas type IV collagen was found only in association with the media of small blood vessels and capillaries in this layer. In the descending thoracic region of the aorta distal to the arch, types I and IV collagens were distributed throughout the intimal and medial layers, whereas type III collagen localization was variable depending on the antibody used for detection. The adventitia stained predominantly for type III collagen that was distinctly organized in folded arrays of fibers. These same fibers stained less intensely with antibody to type I collagen. The abdominal aorta near the common iliacs stained in a fashion similar to that of muscular arteries of the organs and tissues examined, i.e., types I and IV collagen were found in the intimal and medial layers, whereas type III collagen localized heavily to the adventitial layer, with differential staining in the intima and media depending on the antibody used. Additional staining of the adventitial region of the abdominal aorta with type I and type IV collagen antibodies was the same as in the proximal and descending regions. Since the abdominal aorta sustains decreased levels of pulsatile distension compared with the ascending thoracic aorta, type III collagen distribution and its association with other matrix components may be important in regulating the range of distensibility of the vessel wall.