Biocompatibility of lipid-protein-sugar particles containing bupivacaine in the epineurium
Biocompatibility of lipid-protein-sugar particles containing bupivacaine in the epineurium
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DOI：
10.1002/jbm.1261
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发表时间：
2002-03-05
期刊：
影响因子：
--
通讯作者：
Langer, R
中科院分区：
文献类型：
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作者：
Kohane, DS;Lipp, M;Langer, R
新型脂质-蛋白质-糖颗粒（LPSP）具有潜在的生物相容性，因为它们由天然存在的成分组成，并且它们的预期组织停留时间相对较短。在这项研究中，我们使用组织学切片来研究LPSP（4.4 μ m中位直径）用于大鼠坐骨神经阻滞时的组织反应。作为参考，我们将LPSP与60 μ m中位直径的聚（乳酸-乙醇酸）（PLGA）微球（110，000 MW PLGA，乙醇酸/乳酸比65：35）进行了比较。注射后4天，两种颗粒类型均在注射物范围内产生急性炎症、邻近组织炎症和肌毒性。不含布比卡因的颗粒未显示出肌肉毒性，并且邻近组织中的炎症减少。在2周时，LPSP引起的炎症几乎消失，而PLGA微球在注射后至少8周才出现异物巨细胞反应。相比之下，3.6 μ m的中位直径，20，000-MW PLGA微球在注射后2周产生了主要的组织细胞反应。总之，本文研究的LPSP和PLGA微球具有优异的生物相容性，但对前者的组织反应持续时间短得多。肌肉毒性和周围组织的炎症主要归因于布比卡因。异物巨细胞可能归因于颗粒大小，而不是对PLGA的特异性反应。(C)John Wiley & Sons，Inc. J Biomed Mater Res 59：450-459，2002.
Novel lipid-protein-sugar particles (LPSPs) are potentially biocompatible because they are composed of naturally occurring ingredients and their expected tissue dwell times are relatively short. In this research, we used histological sections to study tissue reaction to LPSPs (4.4-mum median diameter) when used for sciatic nerve block in the rat. As a reference, we compared LPSPs to 60-mum median diameter poly(lactic-co-glycolic) acid (PLGA) microspheres (110,000 MW PLGA, glycolic/lactic ratio 65:35). Four days after injection, both particle types produced acute inflammation within the confines of the injectate, inflammation in adjacent tissues, and myotoxicity. Bupivacaine-free particles did not display myotoxicity, and inflammation in adjacent tissues was reduced. At 2 weeks, inflammation from LPSPs had almost disappeared, whereas PLGA microspheres had a foreign-body giant cell reaction until at least 8 weeks after injection. In contrast, 3.6-mum median diameter, 20,000-MW PLGA microspheres produced a primarily histiocytic reaction 2 weeks after injection. In summary, the LPSPs and PLGA microspheres studied herein have excellent biocompatibility, but tissue reaction to the former is of much shorter duration. Myotoxicity and inflammation of surrounding tissue is largely attributed to bupivacaine. Foreign-body giant cells may be attributed to particle size rather than a specific reaction to PLGA. (C) 2001 John Wiley & Sons, Inc. J Biomed Mater Res 59: 450-459, 2002.