Enzyme histochemical analysis of Meckel's cartilage.
Enzyme histochemical analysis of Meckel's cartilage.
复制标题
梅克尔软骨的酶组织化学分析。
作者：
G. Granström;G. Zellin;B. Magnusson;H. Mångs
用组织学和组织化学方法研究了胚胎和新生大鼠下颌骨体的成骨作用，以确定Meckel‘s软骨在骨形成中的作用。Meckel‘s软骨的乳酸脱氢酶和NADH2-黄递酶活性较强，酸性磷酸酶活性较弱，提示软骨具有正常的柠檬酸循环、磷酸戊糖分流和厌氧代谢能力。这些酶的活性在Meckel‘s软骨肥大后下降。碱性磷酸酶是下颌骨类骨质矿化的主要酶，存在于成骨细胞和骨祖细胞中，但不存在于Meckel‘s软骨中。在Meckel‘s软骨和膜内骨分化后，Meckel’s软骨通过下颌骨前部的软骨内成骨支持下颌骨的形成。
Osteogenesis of the body of the mandible in embryonic and neonatal rats was studied histologically and by histochemistry to determine the role of Meckel's cartilage in bone formation. Meckel's cartilage showed intense activity of lactate dehydrogenase and NADH2-diaphorase and weak activity of acid phosphatase, indicating a functioning citric acid cycle, pentose phosphate shunt and a capacity for anaerobic metabolism. The activity of these enzymes declined after hypertrophy of Meckel's cartilage. Alkaline phosphatase was the major enzyme of mineralising mandibular osteoid and was present in the osteoblasts and osteoprogenitor cells but not in Meckel's cartilage. After the differentiation of Meckel's cartilage and intramembranous bone, Meckel's cartilage supported mandibular bone formation by endochondral ossification in the anterior part of the mandible.