Plant mitochondrial RNA editing
Plant mitochondrial RNA editing
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DOI：
10.1007/pl00006473
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发表时间：
1999-03-01
影响因子：
3.9
通讯作者：
Knoop, V
中科院分区：
文献类型：
--
作者：
Steinhauser, S;Beckert, S;Knoop, V
RNA编辑通过种子和非种子植物中胞苷和尿苷碱基的位点特异性交换影响植物线粒体中的信使RNA和转移RNA。这种现象在苔藓中的分布尚不清楚，因为RNA编辑在一些但不是所有的苔类和苔藓中被检测到。要更详细地了解植物中的RNA编辑，需要扩展所研究的分类群和序列的数据集。为了达到这个目的的线粒体nad 5基因（1104 nt）的内部区域进行了分析，在一个大的收集bratellites和绿色藻类（轮藻目）。基因组nad 5序列预测编辑在30藓类，2角苔，和7个简单的叶状体和叶苔（Jungermanniidae）。然而，在七种复杂的叶状苔类（地钱科）和藻类中不需要编辑。另一方面，Jungermanniidae中的RNA编辑频率高达6%的密码子被修改。从基因组序列中RNA编辑的可预测性通过苔藓Schistostega pennata和Rhodobryum roseum，角苔Anthoceros husnotii和A. punctatus和苔类Metzgeria conjugata和Moerckia flotoviana。证实了所有预测重建保守密码子的C-到-U核苷酸交换。金鱼藻中的编辑包括通过频繁的反向U-to-C编辑去除基因组终止密码子。预期的是，通过cDNA分析在地钱科苔类植物Ricciocarpos natans、Corsinia coriandra和Lunularia cruciata中没有鉴定出RNA编辑事件。研究结果进行了讨论，在陆地植物的生育模型。
RNA editing affects messenger RNAs and transfer RNAs in plant mitochondria by site-specific exchange of cytidine and uridine bases in both seed and nonseed plants. Distribution of the phenomenon among bryophytes has been unclear since RNA editing has been detected in some but not all liverworts and mosses. A more detailed understanding of RNA editing in plants required extended data sets for taxa and sequences investigated. Toward this aim an internal region of the mitochondrial nad5 gene (1104 nt) was analyzed in a large collection of bryophytes and green algae (Charales). The genomic nad5 sequences predict editing in 30 mosses, 2 hornworts, and 7 simple thalloid and leafy liverworts (Jungermanniidae). No editing is, however, required in seven species of the complex thalloid liverworts (Marchantiidae) and the algae. RNA editing among the Jungermanniidae, on the other hand, reaches frequencies of up to 6% of codons being modified. Predictability of RNA editing from the genomic sequences was confirmed by cDNA analysis in the mosses Schistostega pennata and Rhodobryum roseum, the hornworts Anthoceros husnotii and A. punctatus, and the liverworts Metzgeria conjugata and Moerckia flotoviana. All C-to-U nucleotide exchanges predicted to reestablish conserved codons were confirmed. Editing in the hornworts includes the removal of genomic stop codons by frequent reverse U-to-C edits. Expectedly, no RNA editing events were identified by cDNA analysis in the marchantiid liverworts Ricciocarpos natans, Corsinia coriandra, and Lunularia cruciata. The findings are discussed in relation to models on the phylogeny of land plants.