High Prevalence of oqxAB in Escherichia coli Isolates from Domestic and Wild Lagomorphs in Italy
High Prevalence of oqxAB in Escherichia coli Isolates from Domestic and Wild Lagomorphs in Italy
复制标题
DOI：
10.1089/mdr.2013.0141
复制
发表时间：
2014-04-01
影响因子：
2.6
通讯作者：
Piccirillo, Alessandra
中科院分区：
文献类型：
--
作者：
Dotto, Giorgia;Giacomelli, Martina;Piccirillo, Alessandra
本研究的目的是确定和表征类1和2整合子和质粒介导的喹诺酮类耐药（PMQR）基因的113多药耐药（MDR）大肠埃希氏菌的收集从农场和野生兔形目动物在2006年和2008年之间在北方意大利。通过琼脂纸片扩散法和粘菌素（COL）E-试验检查菌株的抗菌药物敏感性;通过实时聚合酶链反应（PCR）和DNA测序检查整合子和基因盒含量;通过PCR和DNA测序检查PMQR基因;通过多位点序列分型检查克隆相关性;通过基于PCR的复制子分型检查质粒。在69个分离株（47只家兔、14只野兔和8只野兔）中检测到1类整合子。未发现第2类整合子，共鉴定出5种不同的基因盒阵列（aadA 1、dfrA 1-aadA 1、orfin 682-dhfrA 5、orfin 682-dfrA 5-orfDins 21和dfrA 17-aadA 5）。15%（17/113）的分离株携带oqxAB，没有其他PMQR决定簇。除了一个oqxAB阳性E.大肠杆菌菌株从农场兔子中回收。17株菌株中有7株与主要的ST 238相关，并携带3至6种不同的质粒类型，如IncF、IncHI 1、IncI 1、IncN、IncP、IncX 1、IncY和科尔。6/113株（5.3%）对COL耐药。本研究为了解oqxAB在大肠杆菌中的耐药表型和流行及传播提供了新的见解。这表明这些动物可能是这些遗传决定因子在意大利的储存库，因此是PMQR E的潜在来源。对人类来说是大肠杆菌oqxAB介导的PMQR以前在农场和野生兔形目动物中未检测到。
This study aimed to identify and characterize class 1 and 2 integrons and plasmid-mediated quinolones resistance (PMQR) genes in a collection of 113 multidrug resistance (MDR) Escherichia coli isolated from farm and wild lagomorphs between 2006 and 2008 in Northern Italy. Strains were examined for antimicrobial susceptibility by agar disk diffusion method and E-test for colistin (COL); integrons and gene cassettes content by real-time polymerase chain reaction (PCR) and DNA sequencing; PMQR genes by PCR and DNA sequencing; clonal relatedness by multilocus sequence typing; and plasmids by PCR-based replicon typing. Class 1 integrons were detected in 69 isolates (47 farm rabbits, 14 wild rabbits, and 8 wild hares). No class 2 integrons were found. Five different gene cassettes arrays were identified (aadA1, dfrA1-aadA1, orf in682-dhfrA5, orf in682-dfrA5-orfD ins21, and dfrA17-aadA5). Fifteen percent (17/113) of isolates carried oqxAB, no other PMQR determinants. All but one oqxAB-positive E. coli strains were recovered from farm rabbits. Seven out of 17 strains were associated with the predominant ST238 and carried from three to six different plasmid types, such as IncF, IncHI1, IncI1, IncN, IncP, IncX1, IncY, and ColE. COL resistance was identified in 6/113 strains (5.3%). This study provides new insights on the resistance phenotypes and the prevalence and dissemination of oqxAB in E. coli from farm and wild lagomorphs, suggesting that these animals may be reservoir of these genetic determinants in Italy and thus a potential source of PMQR E. coli for humans. PMQR mediated by oqxAB has not been detected in farm and wild lagomorphs before.