Scratching behavior in NC/Nga mice with dermatitis: Involvement of histamine-induced itching
Scratching behavior in NC/Nga mice with dermatitis: Involvement of histamine-induced itching
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DOI：
10.1111/j.1440-1592.2004.00358.x
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发表时间：
2004-12-01
影响因子：
6.8
通讯作者：
Arai, Iwao
中科院分区：
文献类型：
--
作者：
Hashimoto, Yuki;Takano, Norikazu;Arai, Iwao
背景：瘙痒是包括特应性皮炎（AD）在内的皮炎的典型症状，导致抓痕。在本研究中，我们研究了组胺诱导的瘙痒是否参与NC/Nga （NC）小鼠的抓挠行为，在NC/Nga小鼠中，ad样皮炎是自发诱导的。方法：观察大鼠皮炎严重程度评分、抓痕次数、组胺含量、组胺形成酶组氨酸脱羧酶（HDC）活性、肥大细胞数量及组胺受体拮抗剂作用。结果：我们发现NC小鼠有两种抓痕行为，一种是持续时间短（0.3 ~ 1.0 s），另一种是持续时间长（1.0 s以上）。在常规环境下饲养的NC小鼠，短抓次数和HDC活性随年龄（4-13周）的增加而增加，与皮炎的严重程度平行。持续时间较长的抓挠次数在任何明显的皮炎发展之前已经增加，这种抓挠行为在13周时增加，皮肤中的组胺含量和肥大细胞数量也增加了。在特定的无病原体环境中饲养的NC小鼠，这些参数在实验期间没有变化。氯苯那敏（一种组胺H，受体拮抗剂），而组胺H2和H3/4受体拮抗剂，减少短抓次数。组胺受体拮抗剂没有减少长抓挠的次数，而他克莫司减少了短抓挠和长抓挠的行为。结论：本研究结果表明，HDC活性增强产生的组胺参与了NC小鼠短抓的诱导。
Background: Itching, a typical symptom in dermatitis, including atopic dermatitis (AD), leads to scratching. In the present study, we investigated whether histamine-induced itching is involved in scratching behavior seen in NC/Nga (NC) mice, in which AD-like dermatitis is induced spontaneously. Methods: The severity score of dermatitis, the number of scratches, histamine content, activity of the histamine-forming enzyme histidine decarboxylase (HDC), the number of mast cells and the effect of histamine receptor antagonists were examined. Results: We found two types of scratching behavior in NC mice, one of a short duration (0.3-1.0 s) and the other of a longer duration (over 1.0 s). The number of short scratchings and HDC activity increased with age (4-13 weeks) in parallel with the severity of dermatitis in NC mice raised in conventional surroundings. The number of longer-duration scratchings had increased before any apparent development of dermatitis and this type of scratching behavior increased at 13 weeks, as did histamine content and mast cell number in the skin. There were no changes in these parameters in NC mice raised in specific pathogen-free surroundings during the experimental period. Chlorpheniramine (a histamine H, receptor antagonist), but not histamine H2 and H3/4 receptor antagonists, decreased the number of short scratchings. None of the histamine receptor antagonists decreased the number of long scratchings, whereas tacrolimus reduced both short and long scratching behavior. Conclusions: The findings of the present suggest that histamine produced by enhanced HDC activity is involved in the induction of short scratchings in NC mice.