The regenerating skeletal muscle niche drives satellite cell return to quiescence.
The regenerating skeletal muscle niche drives satellite cell return to quiescence.
复制标题
再生的骨骼肌生态位驱动卫星细胞恢复静止。
DOI：
10.1016/j.isci.2022.104444
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发表时间：
2022-06-17
期刊：
影响因子：
5.8
通讯作者：
Olwin, Bradley B.
中科院分区：
文献类型：
--
作者：
Cutler, Alicia A.;Pawlikowski, Bradley;Wheeler, Joshua R.;Dalla Betta, Nicole;Elston, Tiffany;O'Rourke, Rebecca;Jones, Kenneth;Olwin, Bradley B.
骨骼肌干细胞或卫星细胞（SC）对于再生和维持肌肉至关重要。静止的 SC 存在于基底层和肌纤维膜之间的不对称生态位中。为了修复肌肉，SC 会激活、增殖和分化，融合以修复肌纤维或重新获得静止以补充 SC 生态位。关于干细胞在再生过程中何时重新获得静止或指导干细胞命运决定的细胞过程知之甚少。我们发现，在大多数细胞分化和融合以再生肌纤维后，大多数 SC 在肌肉损伤后 5-10 天重新获得静止。对再生肌肉中的肌原细胞进行单细胞测序，识别出重新获得静止状态的 SC，并揭示非细胞自主信号网络在再生过程中影响 SC 的命运决定。 SC移植实验证实再生环境影响SC的命运。我们定义了生态位 SC 重新填充的窗口，强调再生肌肉环境对 SC 命运的时间贡献。卫星细胞主要在受伤后的前 4 天内产生肌核 大多数卫星细胞在受伤后 5-7 天重新获得静止 重新获得静止的卫星细胞中不对称分裂途径增强 再生的肌肉环境指导卫星细胞的命运决定 细胞生物学；干细胞研究；组学；转录组学。
Skeletal muscle stem cells, or satellite cells (SCs), are essential to regenerate and maintain muscle. Quiescent SCs reside in an asymmetric niche between the basal lamina and myofiber membrane. To repair muscle, SCs activate, proliferate, and differentiate, fusing to repair myofibers or reacquiring quiescence to replenish the SC niche. Little is known about when SCs reacquire quiescence during regeneration or the cellular processes that direct SC fate decisions. We find that most SCs reacquire quiescence 5–10 days after muscle injury, following differentiation and fusion of most cells to regenerate myofibers. Single-cell sequencing of myogenic cells in regenerating muscle identifies SCs reacquiring quiescence and reveals that noncell autonomous signaling networks influence SC fate decisions during regeneration. SC transplantation experiments confirm that the regenerating environment influences SC fate. We define a window for SC repopulation of the niche, emphasizing the temporal contribution of the regenerative muscle environment on SC fate. Satellite cells primarily produce myonuclei in the first 4 days following injury Most satellite cells reacquire quiescence 5–7 days following injury Asymmetric division pathways are elevated in satellite cells reacquiring quiescence The regenerating muscle environment directs satellite cell fate decisions Cell biology; Stem cells research; Omics; Transcriptomics.
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DOI：
10.1242/dev.100842
发表时间：
2014-04
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Chakkalakal JV;Christensen J;Xiang W;Tierney MT;Boscolo FS;Sacco A;Brack AS
通讯作者：
Brack AS
影响因子：
4.6
作者：
Lepper, Christoph;Partridge, Terence A.;Fan, Chen-Ming
通讯作者：
Fan, Chen-Ming
影响因子：
5.2
作者：
Collins, Charlotte A.;Zammit, Peter S.;Partridge, Terence A.
通讯作者：
Partridge, Terence A.
影响因子：
11.8
作者：
Conboy, IM;Rando, TA
通讯作者：
Rando, TA
影响因子：
48
作者：
Browaeys, Robin;Saelens, Wouter;Saeys, Yvan
通讯作者：
Saeys, Yvan