Gas2, a growth arrest-specific protein, is a component of the microfilament network system.
Gas2, a growth arrest-specific protein, is a component of the microfilament network system.
复制标题
GAS2是一种生长逮捕特异性蛋白，是微丝网络系统的组成部分。
DOI：
10.1083/jcb.117.6.1251
复制
发表时间：
1992-06
影响因子：
7.8
通讯作者：
Schneider, C
中科院分区：
文献类型：
--
作者：
Brancolini, C;Bottega, S;Schneider, C
在这篇报告中，我们分析了生长停滞特异性基因gas2的蛋白质产物，通过一种亲和纯化的抗体来对抗细菌中产生的蛋白质。Gas2生物合成的调控反映了mRNA的表达模式（Schneider， C.， R. King, and L. Philipson. 1988）。细胞。54:787-793)：其相对水平与生长停滞密切相关。Gas2似乎也通过磷酸化机制在翻译后水平受到调节。Gas2在进化过程中具有良好的保守性，在小鼠和人之间具有相同的表观分子质量（36kd）。我们还证明了Gas2是微丝系统的一个组成部分。在生长受阻的NIH 3T3细胞中，它与肌动蛋白纤维在细胞边界和沿着应激纤维共定位。在阻滞细胞中检测到的分布模式，也可以在生长细胞中观察到，当它们被微注射纯化的GST-Gas2蛋白时。在所分析的癌基因转化的NIH 3T3细胞系中，血清饥饿均未诱导Gas2表达。
In this report we analyze the protein product of a growth arrest- specific gene, gas2, by means of an affinity-purified antibody raised against the protein produced in bacteria. The regulation of Gas2 biosynthesis reflects the pattern of mRNA expression (Schneider, C., R. King, and L. Philipson. 1988. Cell. 54:787-793): its relative level is tightly associated with growth arrest. Gas2 seems to be regulated also at the posttranslational level via a phosphorylation mechanism. Gas2 is well conserved during the evolution with the same apparent molecular mass (36 kD) between mouse and human. We also demonstrate that Gas2 is a component of the microfilament system. It colocalizes with actin fiber, at the cell border and also along the stress fiber, in growth- arrested NIH 3T3 cells. The pattern of distribution, detected in arrested cells, can also be observed in growing cells when they are microinjected with the purified GST-Gas2 protein. In none of the analyzed oncogene-transformed NIH 3T3 cell lines was Gas2 expression induced under serum starvation.