Golgi inheritance in small buds of Saccharomyces cerevisiae is linked to endoplasmic reticulum inheritance
Golgi inheritance in small buds of Saccharomyces cerevisiae is linked to endoplasmic reticulum inheritance
复制标题
DOI：
10.1073/pnas.0408256102
复制
发表时间：
2004-12-28
影响因子：
11.1
通讯作者：
Glick, BS
中科院分区：
文献类型：
--
作者：
Reinke, CA;Kozik, P;Glick, BS
根据池成熟假说，内质网（ER）衍生膜形成新的高尔基池核。酿酒酵母提供了一个独特的机会来测试这个想法，因为小芽在细胞周期的早期包含内质网和高尔基结构。我们之前预测过内质网遗传缺陷的突变体也会出现高尔基遗传缺陷。令人惊讶的是，酿酒葡萄球菌的研究并没有揭示ER和高尔基遗传之间的联系。在这里，我们通过产生突变株来重新审视这个问题，其中许多小芽缺乏可检测的内质网。这些菌株在早期和晚期高尔基池的遗传上也表现出缺陷。引人注目的是，几乎所有缺乏ER的芽也缺乏早期高尔基池。我们的结果与内质网输出的膜与现有高尔基室衍生的囊泡结合以产生新的高尔基池的想法相吻合。这种高尔基遗传的基本机制可能从酵母保存到脊椎动物细胞。
According to the cisternal maturation hypothesis, endoplasmic reticulum (ER)-derived membranes nucleate new Golgi cisternae. The yeast Saccharomyces cerevisiae offers a unique opportunity to test this idea because small buds contain both ER and Golgi structures early in the cell cycle. We previously predicted that mutants defective in ER inheritance also would show defects in Golgi inheritance. Surprisingly, studies of S. cerevisiae have not revealed the expected link between ER and Golgi inheritance. Here, we revisit this issue by generating mutant strains in which many of the small buds are devoid of detectable ER. These strains also show defects in the inheritance of both early and late Golgi cisternae. Strikingly, virtually all of the buds that lack ER also lack early Golgi cisternae. Our results fit with the idea that membranes exported from the ER coalesce with vesicles derived from existing Golgi compartments to generate new Golgi cisternae. This basic mechanism of Golgi inheritance may be conserved from yeast to vertebrate cells.