Diurnal Temperature Range and Chikungunya Virus Infection in Invasive Mosquito Vectors
Diurnal Temperature Range and Chikungunya Virus Infection in Invasive Mosquito Vectors
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DOI：
10.1093/jme/tjx182
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发表时间：
2018-01-01
影响因子：
2.1
通讯作者：
Lounibos, L. Philip
中科院分区：
文献类型：
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作者：
Alto, Barry W.;Wiggins, Keenan;Lounibos, L. Philip
气候强烈影响蚊媒疾病爆发的地理分布和时间。环境温度影响蚊子的表型性状，包括媒介虫媒病毒的感染能力，外源性潜伏期和传播率的变化介导的。然而，大多数实验都是在恒温下进行的。在自然界中，蚊子更有可能经历每天的温度波动。在这里，我们比较传播感染（腿部感染）和唾液感染的埃及伊蚊（L.）（双翅目：蚊科）和白纹伊蚊（Skuse）（双翅目：蚊科）在口服接触美洲出现的亚洲基因型基孔肯雅病毒后，从佛罗里达发现。我们通过实验评估了变温方案对这些伊蚊传播性感染和唾液感染的影响。三种温度制度中的每一种都具有大致相同的平均温度（27-28摄氏度），但昼夜温差（DTR）的大小不同。大DTR为8.0摄氏度（范围23-31摄氏度），小DTR为4.0摄氏度（范围26-30摄氏度），这与7月至10月期间传播风险最高时佛罗里达不同地点的范围接近。恒温设定在27摄氏度。测试三个地理种群的每种蚊子，传播感染的显着影响被检测到的温度制度和地理种群之间的相互作用，无论是Ae。aegypti和Ae.白纹伊蚊有没有显着的治疗效果的温度，地理种群，或温度的地理种群相互作用对唾液感染的蚊子物种。恒温导致Ae唾液中较高的病毒载量。albopictus，而不是Ae.埃及，与温度波动的条件相比。
Climate strongly influences the geographic distribution and timing of mosquito-borne disease outbreaks. Environmental temperature affects phenotypic traits of mosquitoes including vector competence for arboviruses mediated by changes in infection, extrinsic incubation period and in rates of transmission. Most experiments, however, are done at constant temperatures. In nature, mosquitoes are more likely to experience daily fluctuations in temperature. Here we compare disseminated infection (leg infection) and saliva infection of Aedes aegypti (L.) (Diptera: Culicidae) and Aedes albopictus (Skuse) (Diptera: Culicidae) from Florida following oral exposure to an Asian genotype of chikungunya virus emergent in the Americas. We evaluated experimentally the effect of variable temperature regimens on disseminated infection and saliva infection of these Aedes species. Each of three temperature regimes had approximately the same average temperature (27-28 degrees C), but differed in the magnitude of the diurnal temperature range (DTR). The large DTR was 8.0 degrees C (range 23-31 degrees C) and the small DTR was 4.0 degrees C (range 26-30 degrees C) which approximate ranges in different locations of Florida during July-October when risk of transmission is highest. The constant temperature was set at 27 degrees C. Testing three geographic populations of each mosquito species, significant effects on disseminated infection were detected for an interaction between temperature regime and geographic population for both Ae. aegypti and Ae. albopictus. There were no significant treatment effects of temperature, geographic population, or temperature by geographic population interaction on saliva infection for either mosquito species. Constant temperature resulted in a higher viral load in the saliva of Ae. albopictus, but not Ae. aegypti, compared to conditions where the temperature fluctuated.