De novo synaptogenesis induced by GABA in the developing mouse cortex.
De novo synaptogenesis induced by GABA in the developing mouse cortex.
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DOI：
10.1126/science.aaf5206
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发表时间：
2016-09-02
期刊：
影响因子：
--
通讯作者：
Kwon HB
中科院分区：
文献类型：
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作者：
Oh WC;Lutzu S;Castillo PE;Kwon HB
皮质锥体神经元的树突含有混合的兴奋性和抑制性突触。我们研究了调节突触形成和分布的局部机制。本研究发现，在小鼠出生后早期发育过程中，皮层第2/3层锥体神经元树突局部释放γ-氨基丁酸（GABA），可通过激活GABA A型受体和电压门控性钙通道，诱导桥蛋白斑和树突棘的形成。此外，新形成的抑制性和兴奋性突触结构迅速获得功能。双向操纵GABA释放生长抑素阳性的中间神经元增加和减少的数目，桥蛋白斑点和树突棘，分别。这些结果突出了GABA作为局部突触发生元件的非典型功能，其塑造了小鼠皮层神经元回路的早期建立。
Dendrites of cortical pyramidal neurons contain intermingled excitatory and inhibitory synapses. We studied the local mechanisms that regulate the formation and distribution of synapses. We found that local γ-aminobutyric acid (GABA) release on dendrites of mouse cortical layer 2/3 pyramidal neurons could induce gephyrin puncta and dendritic spine formation via GABA type A receptor activation and voltage-gated calcium channels during early postnatal development. Furthermore, the newly formed inhibitory and excitatory synaptic structures rapidly gained functions. Bidirectional manipulation of GABA release from somatostatin-positive interneurons increased and decreased the number of gephyrin puncta and dendritic spines, respectively. These results highlight a noncanonical function of GABA as a local synaptogenic element shaping the early establishment of neuronal circuitry in mouse cortex.