Maternal undernutrition during early to mid-gestation in the ewe results in altered growth, adiposity, and glucose tolerance in male offspring
Maternal undernutrition during early to mid-gestation in the ewe results in altered growth, adiposity, and glucose tolerance in male offspring
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DOI：
10.2527/jas.2005-624
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发表时间：
2007-05-01
影响因子：
3.3
通讯作者：
Nathanielsz, P. W.
中科院分区：
文献类型：
--
作者：
Ford, S. P.;Hess, B. W.;Nathanielsz, P. W.
本研究利用母羊妊娠早期至中期的母体营养不良，以确定宫内生长受限对雄性后代产后生长的影响及其潜在机制。在妊娠第28天至第78天期间，对经产母羊饲喂其营养需求量（NRC，1985）的50%（营养限制）或100%（对照饲喂），然后从第79天至产羔期间，对所有母羊饲喂100%的NRC需求量。营养限制组（n = 9）和对照组（n = 9）母羊所产的雄性羔羊在出生时表现出相似的BW（5.8 vs. 6.0 +/- 0.3 kg）和顶臀长度（53.8 vs. 55.4 +/- 1.0 cm）。在63和250天的出生后的年龄，公绵羊羔羊进行葡萄糖耐量试验，其中推注的葡萄糖静脉注射，以评估葡萄糖和胰岛素浓度的变化。在63日龄静脉注射葡萄糖后，营养限制母羊的羔羊显示出比对照喂养母羊的羔羊更大的葡萄糖曲线下面积（AUC g; 6，281 vs. 5，242 +/- 429; P < 0.05）和胰岛素曲线下面积（AUC i; 21.0 vs. 8.6 +/- 1.9; P < 0.001）。在250日龄时给予葡萄糖后，与对照饲喂母羊的羔羊相比，营养限制母羊的羔羊具有更高的AUC g（7，147 vs. 5，823 +/- 361; P < 0.01），但AUC i较低（6.4 vs. 10.2 +/- 1.9; P = 0.05）。营养限制母羊的羔羊在4月龄时比对照喂养母羊的羔羊重（26.6 vs. 21.8 +/- 2.3 kg; P < 0.05），背膘厚（0.30 vs. 0.21 +/- 0.03 cm，P < 0.05）。在280日龄屠宰时，来自营养限制母羊的羔羊仍然比来自对照喂养母羊的羔羊重，具有更大（P < 0.05）的肾脏和骨盆区脂肪组织量，并且倾向于（P < 0.10）具有减少的LM和半腱肌重量占HCW的百分比。这些数据表明，在绵羊妊娠早期至中期的一次母体营养不良增加了青春期的BW和脂肪沉积，并且在出生体重没有任何变化的情况下葡萄糖摄取失调。
This study utilized maternal undernutrition from early to midgestation in the ewe to determine the impact(s) of intrauterine growth restriction on postpartum growth of male offspring and the potential mechanisms involved. Multiparous ewes were fed 50% (nutrient-restricted) or 100% (control-fed) of their nutrient requirements (NRC, 1985) between d 28 and 78 of gestation, and then all ewes were fed 100% of the NRC requirements from d 79 through lambing. Male lambs born to nutrient-restricted (n = 9) and control-fed (n = 9) ewes exhibited similar BW (5.8 vs. 6.0 +/- 0.3 kg) and crown-rump lengths (53.8 vs. 55.4 +/- 1.0 cm) at birth. At 63 and 250 d of postnatal age, wether lambs were subjected to a glucose tolerance test, in which a bolus of glucose was administered i.v. to evaluate changes in glucose and insulin concentrations. After i.v. glucose administration at 63 d of age, lambs from nutrient-restricted ewes exhibited a greater area under the curve for glucose (AUCg; 6,281 vs. 5,242 +/- 429; P < 0.05) and insulin (AUCi; 21.0 vs. 8.6 +/- 1.9; P < 0.001) than lambs from control-fed ewes. After glucose administration at 250 d of age, lambs from nutrient-restricted ewes had greater AUCg (7,147 vs. 5,823 +/- 361; P < 0.01) but a lower AUCi (6.4 vs. 10.2 +/- 1.9; P = 0.05) than lambs from control-fed ewes. Lambs from nutrient-restricted ewes were heavier (26.6 vs. 21.8 +/- 2.3 kg; P < 0.05) and had more backfat (0.30 vs. 0.21 +/- 0.03 cm, P < 0.05) by 4 mo of age than the lambs from control-fed ewes. At slaughter at 280 d of age, lambs from nutrient-restricted ewes remained heavier than lambs from control-fed ewes, had greater (P < 0.05) amounts of kidney and pelvic-area adipose tissue, and tended (P < 0.10) to have reduced LM and semitendinosus muscle weights as a percentage of HCW. These data demonstrate that a bout of maternal undernutrition during early to midgestation in sheep increased BW and fat deposition during adolescence and dysregulated glucose uptake in the absence of any change in birth weight.