Nitric oxide-loaded chitosan nanoparticles as an innovative antileishmanial platform
Nitric oxide-loaded chitosan nanoparticles as an innovative antileishmanial platform
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DOI：
10.1016/j.niox.2019.09.007
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发表时间：
2019-12-01
影响因子：
3.9
通讯作者：
Ribeiro, Martha S.
中科院分区：
文献类型：
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作者：
Cabral, Fernanda, V;Pelegrino, Milena T.;Ribeiro, Martha S.
利什曼病是一种被忽视的热带疾病，由于目前药物的不良副作用和耐药性发展，需要新的治疗策略。一氧化氮（NO）供体显示出杀死利什曼原虫属的潜力。但是由于它们的不稳定性，它们的使用受到限制。在这项工作中，我们合成，表征，并封装S-亚硝基巯基琥珀酸到壳聚糖纳米粒子（NONP），并研究其对利什曼原虫（利什曼原虫）亚马逊前鞭毛体和细胞内无鞭毛体的活性。还评价了对巨噬细胞的细胞毒性。我们证实了NONP在一次治疗中减少了两种形式的寄生虫。我们还注意到减少寄生虫空泡作为抑制寄生虫生长和解决感染的证据。在巨噬细胞上未检测到实质性细胞毒性。NONP能够对两种L.（L.）亚马逊感染阶段，对巨噬细胞没有毒性，代表了皮肤利什曼病的有希望的纳米平台。
Leishmaniasis is a neglected tropical disease that demands for new therapeutic strategies due to adverse side effects and resistance development promoted by current drugs. Nitric oxide (NO)-donors show potential to kill Leishmania spp. but their use is limited because of their instability. In this work, we synthesize, characterize, and encapsulate S-nitroso-mercaptosuccinic acid into chitosan nanoparticles (NONPs) and investigate their activity on promastigotes and intracellular amastigotes of Leishmania (Leishmania) amazonensis. Cytotoxicity on macrophages was also evaluated. We verified that NONPs reduced both forms of the parasite in a single treatment. We also noticed reduction of parasitophorous vacuoles as an evidence of inhibition of parasite growth and resolution of infection. No substantial cytotoxicity was detected on macrophages. NONPs were able to provide a sustained parasite killing for both L. (L.) amazonensis infective stages with no toxicity on macrophages, representing a promising nanoplatform for cutaneous leishmaniasis.