Real-Time Microscale Temperature Imaging by Stimulated Raman Scattering
Real-Time Microscale Temperature Imaging by Stimulated Raman Scattering
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DOI：
10.1021/acs.jpclett.0c02029
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发表时间：
2020-09-03
影响因子：
5.7
通讯作者：
Fu, Dan
中科院分区：
文献类型：
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作者：
Figueroa, Benjamin;Hu, Ruoqian;Fu, Dan
水溶液的微尺度温度测量对于理解化学和生物系统中局部热生成和耗散的动力学是必不可少的。已经开发了各种各样的荧光探针来以亚微米分辨率映射温度变化，但它们通常遭受与微环境相关的荧光特性相关的不确定性。在这项工作中，我们开发了一种无标记的比率受激拉曼散射（SRS）显微镜技术，通过监测水的O-H拉曼伸缩模式来量化微尺度温度。通过跟踪氢键O-H带和等吸光度带的比例变化，我们可以直接量化的温度在真实的时间的水基环境中没有外源性造影剂。我们演示了由于激光吸收而引起的局部细胞内和细胞外温度变化的实时测量。这种高速非线性光学成像技术具有在化学和生物系统中的生热作用的原位微尺度成像的潜力。
Microscale thermometry of aqueous solutions is essential to understand the dynamics of local heat generation and dissipation in chemical and biological systems. A wide variety of fluorescent probes have been developed to map temperature changes with submicrometer resolution, but they often suffer from the uncertainty associated with microenvironment-dependent fluorescent properties. In this work, we develop a label-free ratiometric stimulated Raman scattering (SRS) microscopy technique to quantify microscale temperature by monitoring the O-H Raman stretching modes of water. By tracking the ratio changes of the hydrogen-bonding O-H band and the isosbestic band, we can directly quantify the temperature of water-based environments in real time without exogenous contrast agents. We demonstrate real-time measurement of localized intracellular and extracellular temperature changes due to laser absorption. This high-speed nonlinear optical imaging technique has the potential for in situ microscale imaging of thermogenesis in both chemical and biological systems.