Self-assembly combining two bioactive peptide-amphiphile molecules into nanofibers by electrostatic attraction
Self-assembly combining two bioactive peptide-amphiphile molecules into nanofibers by electrostatic attraction
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DOI：
10.1021/ja028215r
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发表时间：
2003-06-18
影响因子：
15
通讯作者：
Stupp, SI
中科院分区：
文献类型：
--
作者：
Niece, KL;Hartgerink, JD;Stupp, SI
我们描述了一种新的方法，肽两亲物（PA）的制备，允许PA与不同的生物活性氨基酸序列组合成一个单一的纤维。不透明的充电PA单独合成，然后混合，以产生凝胶的凝胶的网络在生理pH值。透射电子显微镜显示，形成纤维直径约7 nm，几微米长，在这些二聚体系统。基于核磁共振和显微镜，我们认为这些纳米纤维是混合组合物的圆柱形胶束，由于带相反电荷的PA之间的静电吸引而形成。这种自组装的策略可能在细胞治疗中是有用的，可以在没有侵入性手术或体外组织工程的情况下实施。
We describe a new approach to peptide-amphiphile (PA) nanofiber preparation that allows PAs with different bioactive amino acid sequences to be combined into a single fiber. Oppositely charged PAs are synthesized separately and then mixed to produce gels of nanofiber networks at physiological pH. Transmission electron microscopy reveals the formation of fibers approximately 7 nm in diameter and several micrometers long in these dimeric systems. On the basis of NMR and microscopy, we suggest that these nanofibers are cylindrical micelles of mixed composition, formed due to electrostatic attraction between the oppositely charged PAs. This strategy for self-assembly may be useful in cell therapies that can be implemented without invasive surgery or in in vitro tissue engineering.