The significant expression of TRPV3 in nasal polyps of eosinophilic chronic rhinosinusitis
The significant expression of TRPV3 in nasal polyps of eosinophilic chronic rhinosinusitis
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TRPV3在嗜酸性慢性鼻窦炎鼻息肉中的显着表达
DOI：
10.1016/j.alit.2017.04.002
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发表时间：
2017
影响因子：
6.8
通讯作者：
Fujieda S
中科院分区：
文献类型：
--
作者：
Tokunaga T;Ninomiya T;Kato Y;Imoto Y;Sakashita M;Takabayashi T;Noguchi E;Fujieda S
背景：近年来，日本嗜酸性慢性鼻窦炎（ECRS）患者数量呈上升趋势。在ECRS中，鼻息肉在内窥镜鼻窦手术后立即复发。ECRS难治性的分子生物学机制尚不清楚。方法：采用下一代测序技术（RNA-seq）进行全转录组分析，探讨ECRS的分子生物学机制。采用Real-time PCR、免疫组织化学染色、免疫荧光染色验证RNA-seq结果。结果：RNA-seq分析显示，在ECRS鼻息肉中，3个转录本水平显著升高，7个转录本水平显著降低。在编码这些转录本的基因中，TRPV3（瞬时受体电位阳离子通道，亚家族V，成员3）被鉴定为唯一在ECRS鼻息肉中高表达的基因，但该基因的表达此前未在外周血嗜酸性粒细胞中使用DNA微阵列分析检测到。TRPV3是新发现的在ECRS中转录的基因。我们的分析还发现，TRPV3在ECRS鼻息肉的浸润性嗜酸性粒细胞和粘膜上皮中高表达，且术后难治性越严重，TRPV3在鼻息肉中的表达越高。结论：TRPV3可能在ECRS难治性中起一定作用。需要进一步的研究来评估TRPV3在ECRS中的功能。
Background: The number of patients with eosinophilic chronic rhinosinusitis (ECRS) has been increasing in recent years in Japan. In ECRS, nasal polyps recur immediately after endoscopic sinus surgery. The molecular biological mechanism underlying the refractoriness of ECRS is unclear.Methods: Whole-transcriptome analysis with next-generation sequencing (RNA-seq) was conducted to investigate the molecular biological mechanism of ECRS. Real-time PCR, immunohistochemical staining, and immunofluorescence staining were performed to validate the results of RNA-seq.Results: RNA-seq analysis revealed that in the nasal polyps of ECRS, the levels of 3 transcripts were elevated significantly and those of 7 transcripts were diminished significantly. Among the genes encoding these transcripts, TRPV3 (transient receptor potential cation channel, subfamily V, member 3) was identified as the only gene that is highly expressed in ECRS nasal polyps but this gene's expression was not previously detected using DNA microarray analysis in peripheral blood eosinophils. TRPV3 is newly identified here as a gene transcribed in ECRS. Our analysis also revealed that TRPV3 was highly expressed in the infiltrating eosinophils and mucosal epithelium of the nasal polyps of ECRS, and further that the more severe the refractoriness was after surgery, the higher the TRPV3 expression was in nasal polyps.Conclusions: TRPV3 might play a role in the refractoriness of ECRS. Additional studies are required to evaluate the function of TRPV3 in ECRS.