Seven novel species of Acinetobacter isolated from activated sludge
Seven novel species of Acinetobacter isolated from activated sludge
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DOI：
10.1099/ijs.0.02486-0
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发表时间：
2003-07-01
影响因子：
2.8
通讯作者：
Seviour, RJ
中科院分区：
文献类型：
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作者：
Carr, EL;Kämpfer, P;Seviour, RJ
从澳大利亚维多利亚州活性污泥厂分离到13株不动杆菌。早期的16S-23S rDNA基因组指纹图谱和部分16S rDNA序列数据表明，这些分离物可能含有以前未描述的物种。这一观点在这里得到了证实。多相分类学方法包括表型特征、接近完整的16S rDNA序列数据和DNA-DNA杂交分析，支持在这组分离物中可以区分出7个新的基因组种的观点。然而，当用针对不动杆菌属的16S-rRNA靶向探针进行荧光原位杂交(FISH)研究时，所有这些菌株都呈阳性反应。这表明，在早期的鱼类研究中，这些分离株不会被遗漏，因为它们作为聚磷酸盐积累细菌的作用受到了质疑。本报告描述了这些菌株，并建议将它们命名为贝利不动杆菌(模式菌株B2(T)=DSM 14961(T)=CIP 107474(T))，布氏不动杆菌(模式菌株4B02(T)=DSM 14964(T)=CIP 107468(T))，格氏不动杆菌(模式菌株17A04(T)=DSM 14968(T)=CIP 107470(T))，杰氏不动杆菌(模式菌株7N16(T)=DSM 1497(T)=CIP 107465(T))，毛氏不动杆菌(模式菌株AB1110(T)=DSM 14962(T)=CIP 107472 T)，不动杆菌坦度不动杆菌(模式菌株4N13(T)=DSM 14670(T)=CIP 107469(T))和格氏不动杆菌(模式菌株9A01(T)=DSM 14967(T)=CIP 107464(T))。
Thirteen isolates of Acinetobacter were obtained from activated sludge plants in Victoria, Australia. Earlier 16S-23S rDNA genomic fingerprinting and partial 16S rDNA sequence data had suggested that these isolates might contain previously undescribed species. This view was confirmed here. A polyphasic taxonomic approach involving phenotypic characterization, near-complete 16S rDNA sequence data and DNA-DNA hybridization analyses support the view that seven novel genomic species can be differentiated in this group of isolates. However, when fluorescence in situ hybridization (FISH) studies were performed with a 16S-rRNA-targeted probe specific for the genus Acinetobacter, used to identify Acinetobacter in activated sludge plants, all these strains responded positively. This suggests that these isolates would not have been missed in earlier FISH studies where their role as polyphosphate-accumulating bacteria has been questioned. This report describes these isolates and proposes that they be named Acinetobacter baylyi (type strain B2(T) = DSM 14961(T) = CIP 107474(T)), Acinetobacter bouvetii (type strain 4B02(T) = DSM 14964 (T) = CIP 107468(T)), Acinetobacter grimontii (type strain 17A04(T) = DSM 14968(T) = CIP 107470(T)), Acinetobacter tjernbergiae (type strain 7N16(T) = DSM 1497(T) = CIP 107465(T)), Acinetobacter towneri (type strain AB1110(T) = DSM 14962(T) = CIP 107472 T), Acinetobacter tandoii (type strain 4N13(T) = DSM 14670(T) = CIP 107469(T)) and Acinetobacter gerneri (type strain 9A01(T) = DSM 14967(T) = CIP 107464(T)).