Inhibitory effects of ethanol on rat mesangial cell proliferation via protein kinase C pathway
Inhibitory effects of ethanol on rat mesangial cell proliferation via protein kinase C pathway
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DOI：
10.1097/00000374-200203000-00009
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发表时间：
2002-03-01
影响因子：
3.2
通讯作者：
Shigematsu, A
中科院分区：
文献类型：
--
作者：
Segawa, K;Minami, K;Shigematsu, A
大量证据表明，乙醇抑制多种细胞类型的细胞生长和细胞增殖。然而，乙醇是否抑制肾脏系膜细胞（MC）的增殖还没有研究。我们研究了乙醇对培养的大鼠MC细胞增殖的影响。用乙醇（10-200 mM）处理48小时以浓度依赖性方式抑制[H-3]胸苷掺入MC。相同浓度的乙醇也抑制了MC GF 109203 X细胞数量的增加，蛋白激酶C抑制剂氯化白屈菜红碱消除了乙醇的抑制作用;蛋白激酶C激活剂PMA模拟了乙醇的作用。相反，蛋白激酶A抑制剂H-89和蛋白激酶G抑制剂KT 5823都没有任何作用。这些结果表明，乙醇对MC增殖的抑制作用，可能通过激活蛋白激酶C途径。
A large body of evidence has shown that ethanol inhibits the cell growth and cell proliferation in a variety of cell types. However, it has not been studied whether ethanol inhibits the proliferation of mesangial cells (MC) in the kidney. We examined the effects of ethanol on cell proliferation in cultured rat MC. Treatment with ethanol (10-200 mM) for 48 hr inhibited [H-3]thymidine incorporation into MC in a concentration-dependent manner. The same concentrations of ethanol also inhibited the increase in cell number of MC GF109203X and chelerythrine chloride, inhibitors for protein kinase C, eliminated the inhibitory effects of ethanol; and protein kinase C activator, PMA, mimicked the effects of ethanol. In contrast, neither the protein kinase A inhibitor H-89 nor the protein kinase G inhibitor KT5823 had any effect. These findings suggest that ethanol has inhibitory effects on the proliferation of MC, probably via activation of the protein kinase C pathway.