Use of the polymerase chain reaction assay for the detection of Mycobacterium avium subspecies paratuberculosis in blood and liver biopsies from experimentally infected sheep
Use of the polymerase chain reaction assay for the detection of Mycobacterium avium subspecies paratuberculosis in blood and liver biopsies from experimentally infected sheep
复制标题
DOI：
10.1111/j.1751-0813.2000.tb11938.x
复制
发表时间：
2000-09-01
影响因子：
1.1
通讯作者：
Manktelow, BW
中科院分区：
文献类型：
--
作者：
Gwozdz, JM;Thompson, KG;Manktelow, BW
目的评价聚合酶链反应-本研究采用直接PCR法检测14只实验性感染副结核分枝杆菌的绵羊53周内的肝活检标本和血液标本中的副结核分枝杆菌。结果117只绵羊的血液标本中，来自14只实验感染羊的样本中，两个在PCR测定中检测为阳性。两种阳性结果均在2只亚临床感染绵羊中获得，这些绵羊后来在尸检时通过组织学检查证实了副结核病。然而，该检测未能检测到来自其他五只组织学确诊的副结核病绵羊的血液样本中的靶DNA。同样，PCR检测肝活检标本32周后，管理的副结核分枝杆菌只给了两个阳性结果，这两个都是在羊的组织学证据的副结核分枝杆菌。结论PCR检测血液和肝活检标本不提供一个有用的方法诊断副结核分枝杆菌感染的亚临床感染的羊。
Objective To evaluate a polymerase chain reaction-based assay for the detection of Mycobacterium avium subsp paratuberculosis in blood and liver biopsies from subclinically infected sheep.Study design A direct PCR assay for the detection of M a paratuberculosis was applied to liver biopsy specimens and to samples of blood that were sequentially collected over 53 weeks from 14 sheep infected experimentally with the organism.Results Of 117 blood samples from the 14 experimentally infected sheep, two tested positive in the PCR assay. Both positive results were obtained in two subclinically infected sheep that had paratuberculosis later confirmed by histological examination at necropsy. However, the assay failed to detect the target DNA in samples of blood from five other sheep with histologically confirmed paratuberculosis. Similarly, the PCR assay on liver biopsy specimens collected 32 weeks after administration of M a paratuberculosis gave only two positive results, both of which were obtained in sheep with histological evidence of paratuberculosis.Conclusions The PCR assay on blood and liver biopsies does not provide a useful method for the diagnosis of M a paratuberculosis infection in subclinically infected sheep.