Immunohistochemical detection of pregnancy-specific protein (SP1) and placenta-specific tissue proteins (PP5, PP10, PP11 and PP12) in ovarian adenocarcinomas.
Immunohistochemical detection of pregnancy-specific protein (SP1) and placenta-specific tissue proteins (PP5, PP10, PP11 and PP12) in ovarian adenocarcinomas.
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卵巢腺癌中妊娠特异性蛋白 (SP1) 和胎盘特异性组织蛋白 (PP5、PP10、PP11 和 PP12) 的免疫组织化学检测。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
H. Bohn
中科院分区：
文献类型：
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作者：
N. Inaba;H. Ishige;M. Ijichi;N. Satoh;R. Ohkawa;S. Sekiya;S. Shirotake;H. Takamizawa;T. Renk;H. Bohn
采用免疫球蛋白-酶桥法，对15例粘液性囊腺癌和20例浆液性囊腺癌的妊娠特异性β 1-糖蛋白（SP1）和胎盘特异性组织蛋白（PP 5、PP 10、PP 11、PP 12）进行了研究。在某些恶性肿瘤细胞的胞浆中可检测到这些蛋白。粘液性囊腺癌中，SP1阳性率为53.3%，PP 5阳性率为80.0%，PP 10阳性率为20.0%，PP 11阳性率为66.7%，PP 12阳性率为46.7%。在浆液性囊腺癌中，SP1阳性率为35.0%，PP 5阳性率为76.2%，PP 10阳性率为9.5%，PP 11阳性率为57.1%，PP 12阳性率为23.8%。在5个正常卵巢中未检测到蛋白质。用磷酸盐缓冲盐水或正常、非妊娠、非免疫兔血清代替第一种特异性抗血清以及与相应纯化抗原充分吸收的抗血清孵育的所有对照切片对每种蛋白质均呈阴性。的检测率进行了讨论的临床分期和患者的生存率。这项研究表明，这些蛋白，特别是PP 5和PP 11，可能是有用的监测卵巢腺癌患者的标志物，以及他们的早期诊断。
By using an immunoglobulin-enzyme bridge method, pregnancy-specific beta 1-glycoprotein (SP1) and placenta-specific tissue proteins (PP5, PP10, PP11, PP12) were investigated in 15 cases of mucinous cystadenocarcinoma and 20 cases of serous cystadenocarcinoma. These proteins could be detected in the cytoplasm of some malignant cells. In mucinous cystadenocarcinomas, SP1, was present in 53.3% of cases, PP5 in 80.0%, PP10 in 20.0%, PP11 in 66.7% and PP12 in 46.7%. In serous cystadenocarcinomas, SP1 was present in 35.0% of cases, PP5 in 76.2%, PP10 in 9.5%, PP11 in 57.1% and PP12 in 23.8%. No proteins were detected in five normal ovaries. All control sections incubated with phosphate-buffered saline or normal, non-pregnant, non-immune rabbit serum in place of the first specific antiserum as well as with the antisera exhaustively absorbed with the corresponding purified antigens were negative for each protein. The detection rates are discussed in relation to the clinical stages and survival rates of the patients. This study suggests that these proteins, especially PP5 and PP11, may be useful as markers in monitoring patients with ovarian adenocarcinomas as well as for their early diagnosis.