Dual assay for MCLV3 activity reveals structure-activity relationship of CLE peptides
Dual assay for MCLV3 activity reveals structure-activity relationship of CLE peptides
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DOI：
10.1016/j.bbrc.2008.09.139
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发表时间：
2008-12-05
影响因子：
3.1
通讯作者：
Sakagami, Youji
中科院分区：
文献类型：
--
作者：
Kondo, Tatsuhiko;Nakamura, Touko;Sakagami, Youji
十二肽 MCLV3 是一种功能性肽，源自 CLV3 前体蛋白，CLV3 前体蛋白是 CLV1/CLV2 受体复合物的候选配体，可限制茎尖分生组织 (SAM) 中的干细胞群。 MCLV3可以诱导芽和根分生组织消耗，这是过度表达CLV3的转基因植物的典型表型。我们研究了一系列丙氨酸取代的 MCLV3 和相关肽对拟南芥根生长的生物活性。 MCLV3的构效关系(SAR)与气管元件分化抑制因子(TDIF)具有高度相似性。我们还使用氚化的 MCLV3 和过度表达 MCLV3 胞外域的烟草 BY-2 细胞系的膜部分，通过竞争性受体结合测定评估了肽的结合活性。这种双重测定结合了生物学和受体结合测定来评估 MCLV3 相关肽的活性，揭示了 MCLV3 的 SAR，并表明末端残基在发挥其活性方面发挥着关键作用，并且对于与受体 CLV1 的特异性结合非常重要。 (C) 2008 Elsevier Inc. 保留所有权利。
The dodecapeptide MCLV3 is a functional peptide, derived front the CLV3 precursor protein, which is a candidate ligand of the CLV1/CLV2 receptor complex that restricts the stern cell population in the shoot apical meristem (SAM). MCLV3 can induce shoot and root meristem consumption, the typical phenotype of transgenic plants overexpressing CLV3. We investigated the bioactivities of a series of alanine-Substituted MCLV3 and related peptides on the root growth of Arabidopsis. The structure-activity relationship (SAR) of MCLV3 had high similarity with that of tracheary element differentiation inhibitory factor (TDIF). We also evaluated the binding activities of the peptides by a competitive receptor binding assay using tritiated MCLV3 and the membrane fraction of a tobacco BY-2 cell line overexpressing the MCLV3 ectodomain. This dual assay, combining a biological and receptor binding assay for evaluating the activities of MCLV3-related peptides, uncovered the SAR of MCLV3, and indicated that the terminal residues play Critical roles in exerting its activity and are important for specific binding to the receptor, CLV1. (C) 2008 Elsevier Inc. All rights reserved.