PRODUCTION OF AUXOTROPHIC MUTANTS OF NEISSERIA MENINGITIDIS BY NITROUS ACID.
PRODUCTION OF AUXOTROPHIC MUTANTS OF NEISSERIA MENINGITIDIS BY NITROUS ACID.
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通过亚硝酸生产脑膜炎奈瑟菌营养缺陷型突变体。
DOI：
10.1111/apm.1965.63.4.615
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发表时间：
1965
期刊：
影响因子：
--
通讯作者：
S. Lie
中科院分区：
文献类型：
--
作者：
S. Lie
在脑膜炎球菌的基本培养基配方给出后（7），分离这种生物的生化缺陷突变体成为可能。来自该实验室的早期报道（8）描述了利用紫外线（UV）光的诱变效应，随后通过Gorini和Aufman的改进的青霉素技术浓缩诱导的突变体（4）的这种分离。然而，突变体的产量非常低，并且对UV光和青霉素的影响进行了更广泛的研究。在所采用的条件下，很明显UV光对脑膜炎球菌的诱变效应几乎不可检测（14）。此外，在基本培养基中，JWnicillin对生长和非生长的nwningocococci的选择性杀伤作用在该种中比在基本培养基中更不明显。
After the formula of a minimal medium of meningococci was given (7) it became possible to isolate biochemically deficient mutants of this organism. Earlier reports from this laboratory (8) have described such isolations utilizing the mutagenic effect of ultraviolet (UV) light with subsequent concentration of induced mutants by the modified penicillin technique of Gorini &/{auf man (4). The yield of mutants was very low, however, and more extensive investigations on the effect of UV light and penicillin were performed. Under the conditions employed, it lwcamc clear that the mutagenic t• ffect of UV light on meningococci was hardly detectable (14). Furthermore, the selective killing pffect of JWnicillin on growing and non-growing ct'lls of nwningococci in minimal medium is much less pronounced in this species than in