PERFUSED CAPILLARY MORPHOMETRY IN THE SENESCENT BRAIN
PERFUSED CAPILLARY MORPHOMETRY IN THE SENESCENT BRAIN
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DOI：
10.1016/0197-4580(87)90012-1
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发表时间：
1987-05-01
影响因子：
4.2
通讯作者：
WEISS, HR
中科院分区：
文献类型：
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作者：
BUCHWEITZMILTON, E;WEISS, HR
本研究比较了清醒的年轻（8-10月龄）和衰老（28-33月龄）大鼠脑灌注与总毛细血管床中毛细血管形态计量学的定量指标。通过从特定脑区域切下的组织切片的碱性磷酸酶染色来鉴定总毛细血管床。通过微血管中异硫氰酸荧光素（FITC）葡聚糖的存在来鉴定灌注的毛细血管床。平均毛细血管体积分数，Vv（mm 3/mm 3，平均值±。 S.E.M.）为0.040 ±。0.002和0.033 ±。幼年和老年动物的总毛细血管床分别为0.001。这些数值无统计学差异。灌注Vv平均为0.020 ±。0.001和0.017 ±。0.001 mm3/mm-3。年轻大鼠脑平均灌注Vv为50%，衰老大鼠脑平均灌注Vv为49%。年轻和衰老的大脑利用相似比例的“毛细血管储备”。“在年轻动物的大脑中检查的区域中，发现灌注的毛细血管体积/mm 3，Vv和表面积/mm 3，Sv的百分比存在差异，这在老年动物中不存在。其他人在大脑老化过程中注意到的结构和神经化学变化与平均和区域总或灌注脑毛细血管床形态计量学指数的变化无关。然而，百分比灌注的Vv，Sv，长度，Lv和数量，Na，在年轻的大鼠大脑中的差异不存在于衰老的大鼠大脑。
This study compared quantitative indices of capillary morphometry in the perfused vs. total capillary bed of conscious young (8-10 month old) and senescent (28-33 month old) rat brains. The total capillary bed was identified through alkaline phosphatase staining of tissue sections cut from specific brain regions. The perfused capillary bed was identified by the presence of fluorescein isothiocyanate (FITC) dextran in the microvessels. Average capillary volume fraction, Vv (mm3/mm3, mean .+-. S.E.M.) was 0.040 .+-. 0.002 and 0.033 .+-. 0.001 in the total capillary bed of the young and old animals, respectively. These values were not statistically different. Perfused Vv averaged 0.020 .+-. 0.001 and 0.017 .+-. 0.001 mm3/mm-3 in the young and old animals, respectively. Average perfused Vv was 50% in the young and 49% in the senescent rat brains. Young and senescent brains utilize similar proportions of their "capillary reserves." In the areas examined in the brains of young animals, differences were found in the percentage of capillary volume/mm3, Vv, and surface area/mm3, Sv, perfused which were not present in old animals. The structural and neurochemical changes noted by others in the brain during aging were not related to alterations in indices of average and regional total or perfused cerebral capillary bed morphometry. However, differences in percent perfused Vv, Sv, length, Lv, and number, Na, present in the younger rat brains were not present in the senescent rat brains.