Cytoplasmic phylogeny and evidence of cyto-nuclear co-adaptation in Arabidopsis thaliana
Cytoplasmic phylogeny and evidence of cyto-nuclear co-adaptation in Arabidopsis thaliana
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DOI：
10.1111/j.1365-313x.2010.04275.x
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发表时间：
2010-09-01
期刊：
影响因子：
7.2
通讯作者：
Budar, Francoise
中科院分区：
文献类型：
--
作者：
Moison, Michael;Roux, Fabrice;Budar, Francoise
近年来，拟南芥已成为基因组变异和适应研究的模式物种。虽然已经收集了大量关于该物种核基因组多样性的数据，但关于其细胞质多样性的报道很少。我们分析了95份拟南芥叶绿体(Pt)和线粒体(Mt)基因组的多样性，涵盖了拟南芥的大部分地理来源。对pt基因组的4个基因间隔区进行了测序，共鉴定出68个多态位点和65个pt单倍型。开发了几种策略来鉴定14份材料的mt多态。在此基础上进一步开发了15个多态性标记，并对95份材料进行了分析。利用统计简约法，建立了单倍型群体的pt和mt系统发育网络。为了将pT网络生根，还对两个相关的拟南芥属植物--拟南芥和拟南芥的pT基因间隔区进行了测序。Mt和pt的系统发育高度一致，可以合并成一个单一的细胞质系统发育。为了评估拟南芥核质基因组之间是否存在共适应，我们测试了27对互作F-2家系在挑战条件下的萌发能力。我们发现，细胞质供体对一些F-2家系的发芽力有显著影响。
P>In recent years Arabidopsis thaliana has become a model species for genomic variability and adaptation studies. Although impressive quantities of data have been gathered on the nuclear genomic diversity of this species, little has been published regarding its cytoplasmic diversity. We analyzed the diversity of plastid (pt) and mitochondrial (mt) genomes among 95 accessions, covering most Arabidopsis geographic origins. Four intergenic regions of the pt genome were sequenced, and a total of 68 polymorphisms and 65 pt haplotypes were identified. Several strategies were developed to identify mt polymorphisms among a subset of 14 accessions. Fifteen polymorphisms were further developed as PCR-based markers and used to analyze the whole set of 95 accessions. Using statistical parsimony, we built pt and mt phylogenetic networks of haplotype groups. To root the pt network, the pt intergenic regions of two related Arabidopsis species, Arabidopsis lyrata and Arabidopsis arenosa, were also sequenced. The mt and pt phylogenies are highly congruent and could be combined into a single cytoplasmic phylogeny. To estimate whether co-adaptation between nuclear and cytoplasmic genomes exists in A. thaliana, we tested the germination capacity in challenging conditions of 27 pairs of reciprocal F-2 families. We found that the cytoplasm donor had a significant effect on the germination capacity of some F-2 families.