Nucleotide sequence and functional analysis of the tet (M)-carrying conjugative transposon Tn5251 of Streptococcus pneumoniae
Nucleotide sequence and functional analysis of the tet (M)-carrying conjugative transposon Tn5251 of Streptococcus pneumoniae
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DOI：
10.1111/j.1574-6968.2010.02002.x
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发表时间：
2010-07-01
影响因子：
2.1
通讯作者：
Iannelli, Francesco
中科院分区：
文献类型：
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作者：
Santoro, Francesco;Oggioni, Marco R.;Iannelli, Francesco
Tn 916样遗传元件Tn 5251是肺炎链球菌的复合接合转座子（CTn）Tn 5253的一部分，该复合接合转座子（CTn）Tn 5253是最初称为（cat-tet）BM 6001的64.5-kb染色体元件。DNA序列分析表明，Tn 5251全长18 033 bp，包含22个开放阅读框，其中20个开放阅读框具有相同的转录方向。22个ORF中有11个可以进行注释，包括参与整合/切除过程的泰特（M）四环素抗性基因和int和xis。在含Tn 5253的供体与S.肺炎球菌和粪肠球菌。Tn 5251被证明整合在细菌染色体的不同位点。它作为一个功能齐全的CTn，能够独立的接合转移到各种细菌物种，包括S。肺炎链球菌、戈登链球菌、化脓链球菌、无乳链球菌、E. faecalis和枯草芽孢杆菌。Tn 5251的切除产生环状中间体和Tn 5253中的缺失，其水平为每105条染色体1.2个拷贝。
The Tn916-like genetic element Tn5251 is part of the composite conjugative transposon (CTn) Tn5253 of Streptococcus pneumoniae, a 64.5-kb chromosomal element originally called (cat-tet) BM6001. DNA sequence analysis showed that Tn5251 is 18 033-bp long and contains 22 ORFs, 20 of which have the same direction of transcription. Annotation was possible for 11 out of 22 ORFs, including the tet(M) tetracycline resistance gene and int and xis involved in the integration/excision process. Autonomous copies of Tn5251 were generated during matings of Tn5253-containing donors with S. pneumoniae and Enterococcus faecalis. Tn5251 was shown to integrate at different sites in the bacterial chromosome. It behaves as a fully functional CTn capable of independent conjugal transfer to a variety of bacterial species including S. pneumoniae, Streptococcus gordonii, Streptococcus pyogenes, Streptococcus agalactiae, E. faecalis and Bacillus subtilis. The excision of Tn5251 produces a circular intermediate and a deletion in Tn5253 at a level of 1.2 copies per 105 chromosomes.