Optimization of In Vitro Pecan Pollen Germination
Optimization of In Vitro Pecan Pollen Germination
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DOI：
10.21273/hortsci.46.4.571
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发表时间：
2011-04-01
期刊：
影响因子：
1.9
通讯作者：
Conner, Patrick J.
中科院分区：
文献类型：
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作者：
Conner, Patrick J.
通常需要将花粉从1年储存到下一年，以使得能够在山核桃[Carya illinoinensis（Wangenh.）K.科赫育种计划。通常通过使用体外发芽试验来测试储存的花粉的生活力。为此目的，开发了一种体外萌发测试系统，使用玻璃纸小册子为花粉粒提供固体支持。优化的萌发培养基含有5%蔗糖、20%聚乙二醇8000、0.05%Ca（NO3）（2）、0.025%H3BO3和10 mm 2-（N-吗啉代）乙磺酸（pH 6.0）。花粉在发芽试验前应在加湿室中再水化2至4小时。4至24小时的发芽时间产生类似的最终发芽百分比。在-80 ℃下储存的花粉样品的测试表明，山核桃花粉可以储存至少8年而不降低生活力。使用的化学名称：聚乙二醇（PEG）; 2-（N-吗啉代）乙磺酸（MES）。
Storage of pollen from 1 year to the next is often needed to enable desired crosses to be made in a pecan [Carya illinoinensis (Wangenh.) K. Koch] breeding program. Stored pollen is usually tested for viability through the use of in vitro germination tests. An in vitro germination testing system was developed for this purpose using cellophane booklets to provide a solid support for the pollen grains. Optimized germination media contained 5% sucrose, 20% polyethylene glycol 8000, 0.05% Ca(NO3)(2), 0.025% H3BO3, and 10 mm 2-(N-morpholino)ethanesulfonic acid pH 6.0. Pollen should be rehydrated for 2 to 4 h in a humidified chamber before germination testing. A germination time of 4 to 24 h produces similar final germination percentages. Testing of pollen samples stored at -80 degrees C indicates that pecan pollen can be stored for at least 8 years without a decrease in viability. Chemical names used: polyethylene glycol (PEG); 2-(N-morpholino)ethanesulfonic acid (ME S).