Imaging resin infiltration into non-cavitated carious lesions by optical coherence tomography.

Imaging resin infiltration into non-cavitated carious lesions by optical coherence tomography.
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DOI:
10.1016/j.jdent.2017.03.004
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发表时间:
2017-05
影响因子:
4.4
通讯作者:
H. Schneider;Kyung-Jin Park;C. Rueger;D. Ziebolz;F. Krause;R. Haak
H. Schneider;Kyung-Jin Park;C. Rueger;D. Ziebolz;F. Krause;R. Haak
中科院分区:
医学2区
文献类型:
--
作者:
H. Schneider;Kyung-Jin Park;C. Rueger;D. Ziebolz;F. Krause;R. Haak

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目的应用光谱域光学相干断层扫描技术(SD-OCT)观察白斑型龋损的酸蚀过程和树脂渗漏情况。材料与方法采用显微计算机断层扫描(μCT)技术,对4颗预拔出的人磨牙和前磨牙的非空洞性龋损(ICDAS码2)进行验证。每颗牙齿的一个感兴趣区(ROI)由两个牙合-颈部方向的钻孔标记。用SD-OCT对病变进行成像。根据制造商的说明对病变进行渗透(Icon,DMG)。在每个治疗步骤中和浸润液光固化后,用SD-OCT再次成像感兴趣区。通过感兴趣区对牙齿进行切片,并用扫描电子显微镜对切片层进行成像,以与OCT图像进行比较。结果在蚀刻过程中,病变表面形成了大量的气泡。使用OCT,可以看到树脂渗入龋损小体的过程。早期的釉质龋损完全被树脂浸透,而晚期的釉质龋损是不完全的、不均匀的。结论通过优化酸蚀工艺可以提高树脂的浸润率。光学相干断层扫描提供了有关树脂渗透的过程和程度的信息。临床意义建议在树脂渗透之前使用活化酸。
ObjectivesVisualisation of the etching process and resin penetration at white spot carious lesions by spectral domain optical coherence tomography (SD-OCT).Materials and methodsThe non-cavitated carious lesions (ICDAS code 2) of four visually preselected extracted human molars and premolars were verified as enamel lesions by micro computed tomography (μCT). One region of interest (ROI) per tooth was marked by two drill-holes in occlusal-cervical direction. The lesions were imaged by SD-OCT. Lesions were infiltrated (Icon, DMG) according to the manufacturer’s instructions. During each treatment step and after light curing of the infiltrant, the ROIs were imaged again by SD-OCT. Teeth were sectioned through the ROIs and section layers were imaged by scanning electron microscopy in order to compare with the OCT images. The image sequences for etching and infiltration were viewed in time lapse.ResultsDuring the etching process, numerous bubbles formed on the lesion surface. Using OCT, the process of resin penetration into the carious lesion body became visible. The early enamel carious lesion was completely infiltrated by the resin whereas infiltration of the advanced enamel carious lesion was incomplete and inhomogeneous.ConclusionResin infiltration can be increased by optimizing the etching process. Optical coherence tomography provides information about the process and degree of resin infiltration.Clinical significanceActive acid application before resin infiltration is recommendable.