Therapeutic Effect of Adipose-Derived Stem Cells and BDNF-immobilized PLGA Membrane in a Rat Model of Cavernous Nerve Injury
Therapeutic Effect of Adipose-Derived Stem Cells and BDNF-immobilized PLGA Membrane in a Rat Model of Cavernous Nerve Injury
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DOI：
10.1111/j.1743-6109.2012.02760.x
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发表时间：
2012-08-01
影响因子：
3.5
通讯作者：
Lee, Ji Youl
中科院分区：
文献类型：
--
作者：
Piao, Shuyu;Kim, In Gul;Lee, Ji Youl
介绍。海绵体神经损伤是前列腺切除术后勃起功能障碍（ED）的主要原因。干细胞和神经保护疗法是治疗 ED 的有前景的策略。目的。评估脂肪源性干细胞 (ADSC) 和脑源性神经营养因子 (BDNF) 固定化聚乳酸乙醇酸 (PLGA) 膜对前列腺切除术后 ED 大鼠模型海绵体神经的治疗效果。方法。将大鼠随机分为5组:正常组、双侧海绵神经挤压伤(BCNI)组、ADSC组(海绵神经上有ADSCs的BCNI组)、BDNF-膜组(海绵神经上有BDNF/PLGA膜的BCNI组)、ADSC/BDNF-膜组(海绵神经上有BDNF/PLGA膜覆盖的ADSCs的BCNI组)组。 BDNF 在 BDNF/PLGA 多孔膜系统中受控释放 4 周。主要成果措施。术后4周，通过检测海绵体内压（ICP）/平均动脉压（MAP）的比值来评估勃起功能。通过马森三色染色测定平滑肌和胶原蛋白含量。通过免疫染色检测阴茎背神经中神经元一氧化氮合酶（nNOS）的表达。分别通过蛋白质印迹和cGMP测定来定量海绵体的磷酸内皮一氧化氮合酶（eNOS）蛋白表达和环磷酸鸟苷（cGMP）水平。结果。与 BCNI 和其他治疗组相比，ADSC/BDNF 膜组的勃起功能显着提高。与 BCNI 和其他治疗组相比，ADSC/BDNF 膜治疗显着增加平滑肌/胶原比率、nNOS 含量、磷酸 eNOS 蛋白表达和 cGMP 水平。结论。海绵体神经上带有 BDNF 膜的 ADSC 可以改善前列腺切除后 ED 大鼠模型的勃起功能，这可能作为前列腺切除后 ED 的新疗法。 Piao S、Kim IG、Lee JY、Hong SH、Kim SW、Hwang T-K.、Heang S、Lee JH、Ra JC 和 Lee JY。脂肪干细胞和固定 BDNF 的 PLGA 膜对大鼠海绵体神经损伤模型的治疗作用。 《性医学杂志》2012；9：19681979。
Introduction. Cavernous nerve injury is the main reason for post-prostatectomy erectile dysfunction (ED). Stem cell and neuroprotection therapy are promising therapeutic strategy for ED. Aim. To evaluate the therapeutic efficacy of adipose-derived stem cells (ADSCs) and brain-derived neurotrophic factor (BDNF) immobilized Poly-Lactic-Co-Glycolic (PLGA) membrane on the cavernous nerve in a rat model of post-prostatectomy ED. Methods. Rats were randomly divided into five groups: normal group, bilateral cavernous nerve crush injury (BCNI) group, ADSC (BCNI group with ADSCs on cavernous nerve) group, BDNF-membrane (BCNI group with BDNF/PLGA membrane on cavernous nerve) group, and ADSC/BDNF-membrane (BCNI group with ADSCs covered with BDNF/PLGA membrane on cavernous nerve) group. BDNF was controlled-released for a period of 4 weeks in a BDNF/PLGA porous membrane system. Main Outcome Measures. Four weeks after the operation, erectile function was assessed by detecting the ratio of intra-cavernous pressure (ICP)/mean arterial pressure (MAP). Smooth muscle and collagen content were determined by Masson's trichrome staining. Neuronal nitric oxide synthase (nNOS) expression in the dorsal penile nerve was detected by immunostaining. Phospho-endothelial nitric oxide synthase (eNOS) protein expression and cyclic guanosine monophosphate (cGMP) level of the corpus cavernosum were quantified by Western blotting and cGMP assay, respectively. Results. In the ADSC/BDNF-membrane group, erectile function was significantly elevated, compared with the BCNI and other treated groups. ADSC/BDNF-membrane treatment significantly increased smooth muscle/collagen ratio, nNOS content, phospho-eNOS protein expression, and cGMP level, compared with the BCNI and other treated groups. Conclusions. ADSCs with BDNF-membrane on the cavernous nerve can improve erectile function in a rat model of post-prostatectomy ED, which may be used as a novel therapy for post-prostatectomy ED. Piao S, Kim IG, Lee JY, Hong SH, Kim SW, Hwang T-K., Heang S, Lee JH, Ra JC, and Lee JY. Therapeutic effect of adipose-derived stem cells and BDNF-immobilized PLGA membrane in a rat model of cavernous nerve injury. J Sex Med 2012;9:19681979.